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Inflammatory Response Assessment of a Hybrid Tissue-Engineered Heart Valve Leaflet

Annals of Biomedical Engineering volume 41, pages 316–326 (2013)Cite this article

Abstract

Despite substantial research in the past few decades, only slight progress has been made toward developing biocompatible, tissue-engineered scaffolds for heart valve leaflets that can withstand the dynamic pressure inside the heart. Recent progress on the development of hybrid scaffolds, which are composed of a thin metal mesh enclosed by multi-layered tissue, appear to be promising for heart valve engineering. This approach retains all the advantages of biological scaffolds while developing a strong extracellular matrix backbone to withstand dynamic loading. This study aims to test the inflammatory response of hybrid tissue-engineered leaflets based on characterizing the activation of macrophage cells cultured on the surfaces of the tissue construct. The results indicate that integration of biological layers around a metal mesh core—regardless of its type—may reduce the evoked inflammatory responses by THP-1 monocyte-like cells. This observation implies that masking a metal implant within a tissue construct prior to implantation can hide it from the immune system and may improve the implant’s biocompatibility.

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Notes

  1. EPI: end per inch; PPI: pick per inch.

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Acknowledgments

This work is partially supported by a Coulter Translational Research Award (CTRA) by the Wallace H. Coulter Foundation and a seed grant from the Edwards Lifesciences Center for Advanced Cardiovascular Technology at UC Irvine that was provided to Dr. Kheradvar.

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Authors and Affiliations

  1. The Edwards Lifesciences Center for Advanced Cardiovascular Technology, Department Biomedical Engineering, University of California, Irvine, CA, 92697, USA

    S. Hamed Alavi, Wendy F. Liu & Arash Kheradvar

Authors
  1. S. Hamed Alavi
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  2. Wendy F. Liu
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  3. Arash Kheradvar
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Correspondence to Arash Kheradvar.

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Associate Editor Jane Grande-Allen oversaw the review of this article.

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Alavi, S.H., Liu, W.F. & Kheradvar, A. Inflammatory Response Assessment of a Hybrid Tissue-Engineered Heart Valve Leaflet. Ann Biomed Eng 41, 316–326 (2013). https://doi.org/10.1007/s10439-012-0664-7

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  • DOI: https://doi.org/10.1007/s10439-012-0664-7

Keywords

  • Hybrid heart valve
  • THP-1 cell line
  • Inflammatory response
  • Metal mesh scaffold