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Lipids and Collagen Matrix Restrict the Hydraulic Permeability Within the Porous Compartment of Adult Cortical Bone

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Abstract

In vivo the hydraulic permeability of cortical bone influences the transport of nutrients, waste products and signaling molecules, thus influencing the metabolic functions of osteocytes and osteoblasts. In the current study two hypotheses were tested: the presence of (1) lipids and (2) collagen matrix in the porous compartment of cortical bone restricts its permeability. Our approach was to measure the radial permeability of adult canine cortical bone before and after extracting lipids with acetone-methanol, and before and after digesting collagen with bacterial collagenase. Our results showed that the permeability of adult canine cortical bone was below 4.0 × 10−17 m2, a value consistent with prior knowledge. After extracting lipids, permeability increased to a median value of 8.6 × 10−16 m2. After further digesting with collagenase, permeability increased to a median value of 1.4 × 10−14 m2. We conclude that the presence of both lipids and collagen matrix within the porous compartment of cortical bone restricts its radial permeability. These novel findings suggest that the chemical composition of the tissue matrix within the porous compartment of cortical bone influences the transport and exchange of nutrients and waste products, and possibly influences the metabolic functions of osteocytes and osteoblasts.

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Acknowledgments

Funding for this project was provided in part by the Doctoral Dissertation Defense Program of Cleveland State University. We would like to acknowledge the Cleveland Clinic Musculoskeletal Core Center funded in part by a grant from the National Institute of Arthritis and Musculoskeletal and Skin Diseases, No. 1 P30 AR-050953 for microscopic imaging services; the Kansas Lipidomics Research Center for lipid analyses (supported by NSF grants MCB 0455318 and DBI 0521587, NSF EPSCoR grant EPS-0236913 with matching support from the State of Kansas through Kansas Technology Enterprise Corporation and Kansas State University, and NIH Grant P20 RR16475 from the INBRE program of the National Center for Research Resources); and the Molecular Biology Proteomics Facility at the University of Oklahoma Health Sciences Center for amino acid and amino sugar analyses. We are also grateful to Dr. Matt R. Allen, Assistant Professor in the Department of Anatomy & Cell Biology at the Indiana University School of Medicine, for providing high resolution (3-μm) micro-computed tomography images of our bone wafers.

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Authors and Affiliations

  1. Department of Biomedical Engineering, Lerner Research Institute, Cleveland Clinic, ND20, Cleveland, OH, 44195, USA

    Demin Wen, Caroline Androjna, Amit Vasanji & Ronald J. Midura

  2. Department of Chemical and Biomedical Engineering, Cleveland State University, Cleveland, OH, 44115, USA

    Demin Wen & Joanne Belovich

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  1. Demin Wen
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  2. Caroline Androjna
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  5. Ronald J. Midura
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Correspondence to Ronald J. Midura.

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Demin Wen and Caroline Androjna contributed equally as first authors.

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Wen, D., Androjna, C., Vasanji, A. et al. Lipids and Collagen Matrix Restrict the Hydraulic Permeability Within the Porous Compartment of Adult Cortical Bone. Ann Biomed Eng 38, 558–569 (2010). https://doi.org/10.1007/s10439-009-9858-z

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  • DOI: https://doi.org/10.1007/s10439-009-9858-z

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