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Fast Optical Transillumination Tomography with Large-Size Projection Acquisition

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Abstract

Techniques such as optical coherence tomography and diffuse optical tomography have been shown to effectively image highly scattering samples such as tissue. An additional modality has received much less attention: Optical transillumination (OT) tomography, a modality that promises very high acquisition speed for volumetric scans. With the motivation to image tissue-engineered blood vessels for possible biomechanical testing, we have developed a fast OT device using a collimated, noncoherent beam with a large diameter together with a large-size CMOS camera that has the ability to acquire 3D projections in a single revolution of the sample. In addition, we used accelerated iterative reconstruction techniques to improve image reconstruction speed, while at the same time obtaining better image quality than through filtered backprojection. The device was tested using ink-filled polytetrafluorethylene tubes to determine geometric reconstruction accuracy and recovery of absorbance. Even in the presence of minor refractive index mismatch, the weighted error of the measured radius was <5% in all cases, and a high linear correlation of ink absorbance determined with a photospectrometer of R 2 = 0.99 was found, although the OT device systematically underestimated absorbance. Reconstruction time was improved from several hours (standard arithmetic reconstruction) to 90 s per slice with our optimized algorithm. Composed of only a light source, two spatial filters, a sample bath, and a CMOS camera, this device was extremely simple and cost-efficient to build.

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Acknowledgments

We gratefully acknowledge support from the National Institutes of Health, grant R21 HL081308. We would like to thank Zeus, Inc. for providing PTFE tube samples.

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Authors and Affiliations

  1. Department of Biological Engineering, University of Missouri-Columbia, Columbia, MO, 65211, USA

    Hsuan-Ming Huang & Mark A. Haidekker

  2. Driftmier Engineering Center, Faculty of Engineering, University of Georgia-Athens, Athens, GA, 30602, USA

    Jinjun Xia & Mark A. Haidekker

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  1. Hsuan-Ming Huang
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  2. Jinjun Xia
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  3. Mark A. Haidekker
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Correspondence to Mark A. Haidekker.

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Huang, HM., Xia, J. & Haidekker, M.A. Fast Optical Transillumination Tomography with Large-Size Projection Acquisition. Ann Biomed Eng 36, 1699–1707 (2008). https://doi.org/10.1007/s10439-008-9549-1

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  • DOI: https://doi.org/10.1007/s10439-008-9549-1

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