Abstract
Fluorescent imaging with voltage- and/or calcium-sensitive dyes has revolutionized cardiac physiology research. Here we present improved panoramic imaging for optically mapping electrical activity from the entire epicardium of the Langendorff-perfused rabbit heart. Combined with reconstruction of the 3D heart surface, the functional data can be conveniently visualized on the realistic heart geometry. Methods to quantify the panoramic data set are introduced by first describing a simple approach to mesh the heart in regular grid form. The regular grid mesh provides substrate for easy translation of previously available non-linear dynamics methods for 2D array data. It also simplifies the unwrapping of curved three-dimensional surface to 2D surface for global epicardial visualization of the functional data. The translated quantification methods include activation maps (isochrones), phase maps, phase singularity, and electric stimulus-induced virtual electrode polarization (VEP) maps. We also adapt a method to calculate the conduction velocities on the global epicardial surface by taking the curvature of the heart surface into account.
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Bayly P. V., B. H. KenKnight, J. M. Rogers, R. E. Hillsley, R. E. Ideker, W. M. Smith Estimation of conduction velocity vector fields from epicardial mapping data. IEEE Trans. Biomed. Eng. 45(5): 563–571, 1998. doi:10.1109/10.668746
Bray M. A., S. F. Lin, J. P. Wikswo Three-dimensional surface reconstruction and fluorescent visualization of cardiac activation. IEEE Trans. Biomed. Eng. 47(10): 1382–1391, 2000. doi:10.1109/10.871412
Bray M. A., J. P. Wikswo Considerations in phase plane analysis for nonstationary reentrant cardiac behavior. Phys. Rev. E 65(5): 051902, 2002. doi:10.1103/PhysRevE.65.051902
Bray M. A., J. P. Wikswo Use of topological charge to determine filament location and dynamics in a numerical model of scroll wave activity. IEEE Trans. Biomed. Eng. 49 (10): 1086–1093, 2002. doi:10.1109/TBME.2002.803516
Cheng Y., K. A. Mowrey, D. R. V. Wagoner, P. J. Tchou, I. R. Efimov Virtual electrode-induced reexcitation: a mechanism of defibrillation. Circ. Res. 85: 1056–1066, 1999
Efimov I. R., F. Aguel, Y. Cheng, B. Wollenzier, N. Trayanova Virtual electrode polarization in the far field: implications for external field. Am. J. Physiol. Heart Circ. Physiol. 279: H1055–H1070, 2000
Efimov I. R., Y. Cheng, D. R. V. Wagoner, T. Mazgalev, P. J. Tchou Virtual electrode-induced phase singularity: a basic mechanism of defibrillation failure. Circ. Res. 82: 918–925, 1998
Fedorov V. V., I. T. Lozinsky, E. A. Sosunov, E. P. Anyukhovsky, M. R. Rosen, C. W. Balke, I. R. Efimov Application of blebbistatin as an excitation-contraction uncoupler for electrophysiologic study of rat and rabbit heart. Heart Rhythm 4: 619–626, 2007. doi:10.1016/j.hrthm.2006.12.047
Kay M. W., P. M. Amison, J. M. Rogers Three-dimensional surface reconstruction and panoramic optical mapping of large hearts. IEEE Trans. Biomed. Eng. 51 (7): 1219–1229, 2004. doi:10.1109/TBME.2004.827261
Lin S. F., J. P. Wikswo Panoramic optical imaging of electrical propagation in isolated heart. J. Biomed. Opt. 4(2): 200–207, 1999. doi:10.1117/1.429910
Morad M., G. Salama Optical probes of membrane potential in heart muscle. J. Physiol. 292: 267–295, 1979
Niem W. Robust and fast modeling of 3D natural objects from multiple views. Proc. SPIE 2182: 388–397 1994. doi:10.1117/12.171088
Qu F., C. M. Ripplinger, V. P. Nikolski, C. Grimm, I. R. Efimov Three-dimensional panoramic imaging of cardiac arrhythmias in rabbit heart. J. Biomed. Opt. 12(4): 044019, 2007. doi:10.1117/1.2753748
Rogers J. M. Combined phase singularity and wavefront analysis for optical maps of ventricular fibrillation. IEEE Trans. Biomed. Eng. 51(1): 56–65, 2004. doi:10.1109/TBME.2003.820341
Rogers J. M., G. P. Walcott, J. D. Gladden, S. B. Melnick, M. W. Kay Panoramic optical mapping reveals continuations epicardial reentry during ventricular fibrillation in the isolated swine heart. Biophys. J. 92, 1090–1095, 2007. doi:10.1529/biophysj.106.092098
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Lou, Q., Ripplinger, C.M., Bayly, P.V. et al. Quantitative Panoramic Imaging of Epicardial Electrical Activity. Ann Biomed Eng 36, 1649–1658 (2008). https://doi.org/10.1007/s10439-008-9539-3
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DOI: https://doi.org/10.1007/s10439-008-9539-3