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Computation of Adherent Cell Elasticity for Critical Cell-Bead Geometry in Magnetic Twisting Experiments

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Abstract

Quantification of the cell elastic modulus is a central issue of micromanipulation techniques used to analyze the mechanical properties of living adherent cells. In magnetic twisting cytometry (MTC), magnetic beads of radius R, linked to the cell cytoskeleton through transmembrane receptors, are twisted. The relationships between imposed external torque and measured resulting bead rotation or translation only provide values of the apparent cell stiffness. Thus, specific correcting coefficients have to be considered in order to derive the cell elastic modulus. This issue has been highlighted in previous studies, but general relationships for handling such corrections are still lacking while they could help to understand and reduce the large dispersion of the reported values of cell elastic modulus. Thiswork establishes generalized abacuses of the correcting coefficients from which the Young’s modulus of a cell probed byMTCcan be derived. Based on a 3Dfinite element analysis of an hyperelastic (neo-Hookean) cell, we show that the dimensionless ratio hu/2R, where hu is the cell height below the bead, is an essential parameter for quantification of the cell elasticity. This result could partly explain the still intriguing question of the large variation of measured elastic moduli with probe size.

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Authors and Affiliations

  1. Laboratoire TIMC-IMAG, équipe DynaCell, CNRS, UMR 5525, Institut de l’Ingénierie et de l’Information de Santé (In3S), 38706 La Tronche cedex, France

    Jacques Ohayon (Pr) & Philippe Tracqui (Dr)

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  1. Jacques Ohayon
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  2. Philippe Tracqui
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Correspondence to Jacques Ohayon or Philippe Tracqui.

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Ohayon, J., Tracqui, P. Computation of Adherent Cell Elasticity for Critical Cell-Bead Geometry in Magnetic Twisting Experiments. Ann Biomed Eng 33, 131–141 (2005). https://doi.org/10.1007/s10439-005-8972-9

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  • DOI: https://doi.org/10.1007/s10439-005-8972-9

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