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Enzyme-Linked Immunosorbent Assay of Ampicillin in Milk

  • Zh. V. Samsonova
  • O. S. Shchelokova
  • N. L. Ivanova
  • M. Yu. Rubtsova
  • A. M. Egorov
Article

Abstract

An indirect immunoassay for quantitative determination of ampicillin (range, 10–1000 ng/ml) in buffer or milk has been developed. Polyclonal antibodies were obtained against ampicillin conjugated with bovine serum albumin; the conjugate was synthesized by direct condensation using carbodiimide. The antibodies were specific for ampicillin and exhibited low cross-reactivity to other penicillins (azlocillin, 17%; penicillin G, 10%; piperacillin, 5%; and carbenicillin, 4%). Matrix effects were minimized by combining the use of a casein-supplemented buffer (content of casein, 1%) with sample dilution. Limit of detection for ampicillin in milk (diluted tenfold) was equal to 5.0 ng/ml (which corresponded to 50 ng/ml of the original sample).

Keywords

Albumin Bovine Serum Albumin Serum Albumin Penicillin Ampicillin 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© MAIK "Nauka/Interperiodica" 2005

Authors and Affiliations

  • Zh. V. Samsonova
    • 1
  • O. S. Shchelokova
    • 1
  • N. L. Ivanova
    • 1
  • M. Yu. Rubtsova
    • 1
  • A. M. Egorov
    • 1
  1. 1.Faculty of ChemistryMoscow State UniversityRussia

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