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Bioconversion of β-Sitosterol and Its Esters by Actinobacteria of the Genus Rhodococcus

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Abstract

The ability of pure cultures of Rhodococcus actinobacteria from the Ural Specialized Collection of Alkanotrophic Microorganisms (World Federation for Culture Collections accession number 768; http://www.ecology.psu.ru/iegmcol) to convert β-sitosterol (BSS) and its 3β-acylated derivatives was studied. Rhodococcus strains with pronounced cholesterol oxidase activity, capable of converting BSS to stigmat-4-ene-3-one in the reaction of cooxidation with n-hexadecane, were selected. The dependence of the activity of cholesterol oxidase of rhodococci on the length of the acyl group in BSS esters was studied. Conditions under which Rhodococcus cells convert BSS to 17β-hydroxyandrost-4-ene-3-one (testosterone), commonly used in pharmacology, were determined.

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Authors and Affiliations

  1. Institute of Ecology and Genetics of Microorganisms, Ural Division, Russian Academy of Sciences, ul. Goleva 13, Perm, 614081, Russia

    I. B. Ivshina & E. M. Nogovitsina

  2. Institute of Technical Chemistry, Ural Division, Russian Academy of Sciences, ul. Lenina 13, Perm, 614990, Russia

    V. V. Grishko

  3. Institute of Organic Chemistry, Siberian Division, Russian Academy of Sciences, pr. Akademika Lavrent'eva 9, Novosibirsk, 630090, Russia

    T. P. Kukina & G. A. Tolstikov

Authors
  1. I. B. Ivshina
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  2. V. V. Grishko
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  3. E. M. Nogovitsina
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  4. T. P. Kukina
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  5. G. A. Tolstikov
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__________

Translated from Prikladnaya Biokhimiya i Mikrobiologiya, Vol. 41, No. 6, 2005, pp. 626–633.

Original Russian Text Copyright © 2005 by Ivshina, Grishko, Nogovitsina, Kukina, Tolstikov.

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Ivshina, I.B., Grishko, V.V., Nogovitsina, E.M. et al. Bioconversion of β-Sitosterol and Its Esters by Actinobacteria of the Genus Rhodococcus . Appl Biochem Microbiol 41, 551–557 (2005). https://doi.org/10.1007/s10438-005-0100-y

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  • DOI: https://doi.org/10.1007/s10438-005-0100-y

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