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Determination of Minimal Concentrations of Biocorrosion Inhibitors by a Bioluminescence Method

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Abstract

By using a bioluminescence ATP assay, we have determined the minimal concentrations of some biocorrosion inhibitors (Kathon, Khazar, VFIKS-82, Nitro-1, Caspii-2, and Caspii-4) suppressing most common microbial corrosion inducers: Desulfovibrio desulfuricans, Desulfovibrio vulgaris, Pseudomonas putida, Pseudomonas fluorescens, and Acidithiobacillus ferrooxidans. The cell titers determined by the bioluminescence method, including not only fissiparous cells but also their dormant living counterparts, are two-to sixfold greater than the values determined microbiologically. It is shown that the bioluminescence method can be applied to determination of cell titers in samples of oil-field waters in the presence of iron ions (up to 260 mM) and iron sulfide (to 186 mg/l) and in the absence or presence of biocidal corrosion inhibitors.

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Translated from Prikladnaya Biokhimiya i Mikrobiologiya, Vol. 41, No. 4, 2005, pp. 429–434.

Original Russian Text Copyright © 2005 by Efremenko, Azizov, Makhlis, Abbasov, Varfolomeev.

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Efremenko, E.N., Azizov, R.E., Makhlis, T.A. et al. Determination of Minimal Concentrations of Biocorrosion Inhibitors by a Bioluminescence Method. Appl Biochem Microbiol 41, 377–381 (2005). https://doi.org/10.1007/s10438-005-0064-y

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  • DOI: https://doi.org/10.1007/s10438-005-0064-y

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