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Enzyme immunoassay of immune complexes formed in vitro via interactions of serum antibodies with diphtheria toxin

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Abstract

The interaction of diphtheria toxin with serum antitoxin antibodies has been studied by enzyme immunoassay at variable ratios of the original amounts of the antigen and antibodies in the reaction mixture. Under the conditions of excess of the antibodies, the free toxin was not detected, and free antibodies accounted for 68 to 98% of the original amount of the antibodies. Under the conditions of excess of the toxin, free antibodies account for 2 to 7% of the original amount and free toxin, for 80–100% of its original level. Under the conditions where the toxin is taken in excess, and the amounts of the toxin and the antibodies are equivalent, formed immune complexes are regularly detected in the reaction mixtures. In these complexes, part of the epitopes of the toxin remains free from antibodies. The data obtained are interpreted from the viewpoint of epitope heterogeneity, bivalence of serum antibodies, and monovalence of the toxin epitopes. A new model of the toxin-antibody interaction is proposed.

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Authors and Affiliations

  1. Mechnikov Research Institute of Vaccines and Sera, Russian Academy of Medical Sciences, 105064, Moscow, Russia

    N. G. Titova & V. V. Sviridov

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  1. N. G. Titova
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  2. V. V. Sviridov
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Translated from Prikladnaya Biokhimiya i Mikrobiologiya, Vol. 41, No. 2, 2005, pp. 235–242.

Original Russian Text Copyright © 2005 by Titova, Sviridov.

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Titova, N.G., Sviridov, V.V. Enzyme immunoassay of immune complexes formed in vitro via interactions of serum antibodies with diphtheria toxin. Appl Biochem Microbiol 41, 205–212 (2005). https://doi.org/10.1007/s10438-005-0036-2

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  • DOI: https://doi.org/10.1007/s10438-005-0036-2

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