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Activation of peroxidase-catalyzed oxidation of chromogenic substrates by tetrazole and its 5-substituted derivatives

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Abstract

Peroxidase-catalyzed oxidation of 2,2-azino-di(3-ethyl-benzthiazolydine-6-sulfonic acid) (ABTS) and 3,3′,5,5′-tetramethylbenzidine (TMB) is activated by tetrazole and its 5-substituted derivatives—5-amino-(AmT), 5-methyl-(MeT), 5-phenyl-(PhT), and 5-CF3-(CF3-T) tetrazoles. In phosphate-citrate or phosphate buffer (pH 6.4 or 7.2; 20°C), the activating effect of tetrazoles on TMB and ABTS oxidation decreased in the series AmT > MeT > T > PhT > CF3-T and T > AmT > MeT > PhT, respectively. The coefficient (degree) of activation (α), expressed in M−1, determined for both substrates and all activators, depended on substrate type, buffer nature, and pH (it increased as pH increased from 6.4 to 7.2). For TMB oxidation, good correlation between logα and the Hammet constants σmeta for m-substituents in the benzene series NH2, CH3, C6H5, and CF3 was found. It is suggested that AmT, MeT, and T can be used as activators of peroxidase-catalyzed oxidation of TMB and ABTS in enzyme immunoassay and designing peroxidase-based biosensors.

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Abbreviations

A:

Activator

ABTS:

diammonium salt of 2,2-azino-di(3-ethyl-benzthiazolydine-6-sulfonic acid)

TMB:

3,3′,5,5′-tetramethylbenzidine

T:

tetrazole

AmT:

5-aminotetrazole

MeT:

5-methyltetrazole

PhT:

5-phenyltetrazole

CF3-T:

5-CF3-tetrazole

HP:

horseradish peroxidase

PB:

0.06 M phosphate buffer

PCB:

0.06 M phosphate-citrate buffer

α:

coefficient (degree) of activation of peroxidase-catalyzed oxidation of ABTS and TMB

σmeta :

the Hammet constant for meta-substituents in the benzene series

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Authors and Affiliations

  1. Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus, ul. Kuprevicha 5/2, 220141, Minsk, Belarus

    E. I. Karasyova & D. I. Metelitza

  2. Research Institute of Physicochemical Problems, State University of Belarus, ul. Leningradskaya 14, 220050, Minsk, Belarus

    P. N. Gaponik

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  1. E. I. Karasyova
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Translated from Prikladnaya Biokhimiya i Mikrobiologiya, Vol. 41, No. 2, 2005, pp. 148–157.

Original Russian Text Copyright © 2005 by Karasyova, Gaponik, Metelitza.

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Karasyova, E.I., Gaponik, P.N. & Metelitza, D.I. Activation of peroxidase-catalyzed oxidation of chromogenic substrates by tetrazole and its 5-substituted derivatives. Appl Biochem Microbiol 41, 129–138 (2005). https://doi.org/10.1007/s10438-005-0022-8

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