Abstract
This paper reports a fabrication method that can make microstructures such as microfluidic channels and nanostructures to generate surface plasmon resonance (SPR) signals in one-step using hot embossing. We first made a micro/nanostructural mold on a silicon substrate through sequential e-beam lithography, reactive ion etching (RIE), photolithography, and inductively coupled plasma RIE. The fabricated mold and cyclo-olefin polymer (COP) film were pressed between two flat, heated metal bases under optimal conditions, and the micro/nanostructures were complementarily transferred to the COP film. After depositing a thin aluminum film onto the nanostructure, the device was completed by patterning Nafion that crossed two channels and a nearby nanostructure, and by bonding the COP film to a flat polydimethylsiloxane (PDMS) substrate with holes punched for the inlets and outlets. SPR signals of the nanostructures of the microfluidic channel were calibrated using glycerol solutions of different percentages, and a wavelength sensitivity of 393 nm/refractive index unit was found for the Al-based nanoslit SPR sensing chip. To detect macromolecules, we first modified bovine serum albumin (BSA) onto the surface of the SPR chip and then allowed different concentrations of anti-BSA samples to flow into the device. A calibration curve for detecting anti-BSA was constructed, and anti-BSA detection levels with and without preconcentration were compared.
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The data that support the findings of this study are available from the corresponding author upon reasonable request.
References
Chang Y-C, Dvoynenko MM, Ke H et al (2021) Double resonance SERS substrates: Ag nanoparticles on grating. J Phys Chem C 125:27267–27274. https://doi.org/10.1021/acs.jpcc.1c07454
Chuang C-S, Wu C-Y, Juan P-H et al (2020) LMP1 gene detection using a capped gold nanowire array surface plasmon resonance sensor in a microfluidic chip. Analyst 145:52–60. https://doi.org/10.1039/C9AN01419E
Chung P-S, Fan Y-J, Sheen H-J, Tian W-C (2015) Real-time dual-loop electric current measurement for label-free nanofluidic preconcentration chip. Lab Chip. https://doi.org/10.1039/c4lc01143k
Das S, Devireddy R, Gartia MR (2023) Surface plasmon resonance (SPR) sensor for cancer biomarker detection. Biosensors (basel) 13:396. https://doi.org/10.3390/bios13030396
Deng C-Z, Fan Y-J, Chung P-S, Sheen H-J (2018) A novel thermal bubble valve integrated nanofluidic preconcentrator for highly sensitive biomarker detection. ACS Sens 3:1409–1415. https://doi.org/10.1021/acssensors.8b00323
Dutta P, Su T-Y, Fu A-Y et al (2022) Combining portable solar-powered centrifuge to nanoplasmonic sensing chip with smartphone reader for rheumatoid arthritis detection. Chem Eng J 434:133864. https://doi.org/10.1016/j.cej.2021.133864
Ebbesen TW, Lezec HJ, Ghaemi HF et al (1998) Extraordinary optical transmission through sub-wavelength hole arrays. Nature 391:667–669. https://doi.org/10.1038/35570
Fan Y-J, Deng C-Z, Chung P-S et al (2018) A high sensitivity bead-based immunoassay with nanofluidic preconcentration for biomarker detection. Sens Actuators B Chem 272:502–509. https://doi.org/10.1016/j.snb.2018.05.141
Fan Y-J, Huang M-Z, Hsiao Y-C et al (2020) Enhancing the sensitivity of portable biosensors based on self-powered ion concentration polarization and electrical kinetic trapping. Nano Energy 69:104407. https://doi.org/10.1016/j.nanoen.2019.104407
Geng Z, Kan Q, Yuan J, Chen H (2014) A route to low-cost nanoplasmonic biosensor integrated with optofluidic-portable platform. Sens Actuators B Chem 195:682–691. https://doi.org/10.1016/j.snb.2014.01.110
Homola J, Piliarik M (2006) Surface plasmon resonance (SPR) sensors. Springer Ser Chem Sens Biosens 4:45–67. https://doi.org/10.1007/5346_014
Homola J, Yee SS, Gauglitz G (1999) Surface plasmon resonance sensors: review. Sens Actuators B Chem 54:3–15. https://doi.org/10.1016/S0925-4005(98)00321-9
Hsiao H-H, Ke H, Dvoynenko MM, Wang J-K (2020) Multipolar resonances of ag nanoparticle arrays in anodic aluminum oxide nanochannels for enhanced hot spot intensity and signal-to-background ratio in surface-enhanced Raman scattering. ACS Appl Nano Mater 3:4477–4485. https://doi.org/10.1021/acsanm.0c00569
Hsieh H-Y, Chang R, Huang Y-Y et al (2022a) Continuous polymerase chain reaction microfluidics integrated with a gold-capped nanoslit sensing chip for Epstein-Barr virus detection. Biosens Bioelectron 195:113672. https://doi.org/10.1016/j.bios.2021.113672
Hsieh H-Y, Luo J-X, Shen Y-H et al (2022b) A nanofluidic preconcentrator integrated with an aluminum-based nanoplasmonic sensor for Epstein-Barr virus detection. Sens Actuators B Chem 355:131327. https://doi.org/10.1016/j.snb.2021.131327
Jian C, Zhang J, Ma X (2020) Cu–Ag alloy for engineering properties and applications based on the LSPR of metal nanoparticles. RSC Adv 10:13277–13285. https://doi.org/10.1039/D0RA01474E
Ko SH, Song YA, Kim SJ et al (2012) Nanofluidic preconcentration device in a straight microchannel using ion concentration polarization. Lab Chip. https://doi.org/10.1039/c2lc21238b
Kuo T-R, Chen Y-C, Wang C-I et al (2017) Highly oriented Langmuir-Blodgett film of silver cuboctahedra as an effective matrix-free sample plate for surface-assisted laser desorption/ionization mass spectrometry. Nanoscale 9:11119–11125. https://doi.org/10.1039/C7NR04098A
Kwak R, Kim SJ, Han J (2011) Continuous-flow biomolecule and cell concentrator by ion concentration polarization. Anal Chem. https://doi.org/10.1021/ac2012619
Lee K-L, Chen P-W, Wu S-H et al (2012) Enhancing surface plasmon detection using template-stripped gold nanoslit arrays on plastic films. ACS Nano 6:2931–2939. https://doi.org/10.1021/nn3001142
Lee K-L, Huang J-B, Chang J-W et al (2015) Ultrasensitive biosensors using enhanced fano resonances in capped gold nanoslit arrays. Sci Rep 5:8547. https://doi.org/10.1038/srep08547
Lee K-L, You M-L, Tsai C-H et al (2016) Nanoplasmonic biochips for rapid label-free detection of imidacloprid pesticides with a smartphone. Biosens Bioelectron. https://doi.org/10.1016/j.bios.2015.08.010
Lee K-L, You M-L, Wei P-K (2019) Aluminum nanostructures for surface-plasmon-resonance-based sensing applications. ACS Appl Nano Mater 2:1930–1939. https://doi.org/10.1021/acsanm.8b02325
Lezec HJ, Degiron A, Devaux E et al (2002) Beaming light from a subwavelength aperture. Science 297:820–822. https://doi.org/10.1126/science.1071895
Lu Y-J, Hsieh H-Y, Kuo W-C et al (2021) Nanoplasmonic structure of a polycarbonate substrate integrated with parallel microchannels for label-free multiplex detection. Polymers (basel) 13:3294. https://doi.org/10.3390/polym13193294
Mahmoudpour M, Ezzati Nazhad Dolatabadi J, Torbati M et al (2019) Nanomaterials and new biorecognition molecules based surface plasmon resonance biosensors for mycotoxin detection. Biosens Bioelectron 143:111603. https://doi.org/10.1016/j.bios.2019.111603
Masson J-F (2017) Surface plasmon resonance clinical biosensors for medical diagnostics. ACS Sens. https://doi.org/10.1021/acssensors.6b00763
Mostufa S, Akib TBA, Rana MdM, Islam MdR (2022) Highly sensitive TiO2/Au/graphene layer-based surface plasmon resonance biosensor for cancer detection. Biosensors (basel) 12:603. https://doi.org/10.3390/bios12080603
Mousavi M, Chen H-Y, Hou H-S et al (2015) Label-free detection of rare cell in human blood using gold nano slit surface plasmon resonance. Biosensors (basel) 5:98–117. https://doi.org/10.3390/bios5010098
Sheen H-J, Panigrahi B, Kuo T-R et al (2022) Electrochemical biosensor with electrokinetics-assisted molecular trapping for enhancing C-reactive protein detection. Biosens Bioelectron 210:114338. https://doi.org/10.1016/j.bios.2022.114338
Sherry LJ, Chang S-H, Schatz GC et al (2005) Localized surface plasmon resonance spectroscopy of single silver nanocubes. Nano Lett 5:2034–2038. https://doi.org/10.1021/nl0515753
Singh P (2016) SPR biosensors: historical perspectives and current challenges. Sens Actuators B Chem 229:110–130
Soler M, Belushkin A, Cavallini A et al (2017) Multiplexed nanoplasmonic biosensor for one-step simultaneous detection of Chlamydia trachomatis and Neisseria gonorrhoeae in urine. Biosens Bioelectron 94:560–567. https://doi.org/10.1016/j.bios.2017.03.047
Stewart ME, Anderton CR, Thompson LB et al (2008) Nanostructured plasmonic sensors. Chem Rev 108:494–521. https://doi.org/10.1021/cr068126n
Sui M, Kunwar S, Pandey P, Lee J (2019) Strongly confined localized surface plasmon resonance (LSPR) bands of Pt, AgPt, AgAuPt Nanoparticles. Sci Rep 9:16582. https://doi.org/10.1038/s41598-019-53292-1
Tan S-H, Yougbaré S, Tao H-Y et al (2021) Plasmonic gold nanoisland film for bacterial theranostics. Nanomaterials 11:3139. https://doi.org/10.3390/nano11113139
Wang YC, Stevens AL, Han J (2005) Million-fold preconcentration of proteins and peptides by nanofluidic filter. Anal Chem. https://doi.org/10.1021/ac050321z
Wang S-H, Lo S-C, Tung Y-J et al (2020) Multichannel nanoplasmonic platform for imidacloprid and fipronil residues rapid screen detection. Biosens Bioelectron 170:112677. https://doi.org/10.1016/j.bios.2020.112677
Xu T, Geng Z (2021) Strategies to improve performances of LSPR biosensing: Structure, materials, and interface modification. Biosens Bioelectron 174:112850. https://doi.org/10.1016/j.bios.2020.112850
Yougbaré S, Mutalik C, Krisnawati DI et al (2020) Nanomaterials for the photothermal killing of bacteria. Nanomaterials 10:1123. https://doi.org/10.3390/nano10061123
Yougbaré S, Chou H-L, Yang C-H et al (2021) Facet-dependent gold nanocrystals for effective photothermal killing of bacteria. J Hazard Mater 407:124617. https://doi.org/10.1016/j.jhazmat.2020.124617
Yu X, Liu X, Wang B et al (2020) An LSPR-based “push–pull” synergetic effect for the enhanced photocatalytic performance of a gold nanorod@cuprous oxide-gold nanoparticle ternary composite. Nanoscale 12:1912–1920. https://doi.org/10.1039/C9NR08808C
Zhao J, Zhang X, Yonzon CR et al (2006) Localized surface plasmon resonance biosensors. Nanomedicine 1:219–228
Zhou J, Qi Q, Wang C et al (2019) Surface plasmon resonance (SPR) biosensors for food allergen detection in food matrices. Biosens Bioelectron 142:111449
Acknowledgements
This work was supported by the National Science and Technology Council of Taiwan (grant nos. NSTC 112-2636-E-038-003, 111-2221-E-002-128, and 111-2811-E-002-030), and by Taipei Medical University Hospital (grant no. 112TMU-TMUH-07). We would also like to thank the NEMS Research Center, National Taiwan University for facility support.
Funding
Taipei Medical University Hospital, 112TMU-TMUH-07, National Science and Techology Council of Taiwan, NSTC 111-2811-E-002 -030, NSTC 111-2221-E-002 -128, NSTC 112-2636-E-038 -003.
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Y-JL, H-JS, Y-JF fund the project. Y-JL, and H-YH analyze the data, and draft the manuscript. W-FY collected the data. K-CW, HT provided the idea and proof the analyzed data. P-KW provided the idea and technique of fabrication. H-JS and Y-J Fan provide the fabrication skill, and finalized the manuscript. All authors reviewed the manuscript.
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Lu, YJ., Hsieh, HY., Yang, WF. et al. Co-printing of micro/nanostructures integrated with preconcentration to enhance protein detection. Microfluid Nanofluid 28, 3 (2024). https://doi.org/10.1007/s10404-023-02699-4
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DOI: https://doi.org/10.1007/s10404-023-02699-4