Diffusion plays a critical role in establishing functional bio/solid soft interfaces for bioassays, biosensors, and biofuel cells. An understanding of micro-diffusion near the interface is significant for developing high-performance bioassays, biosensors, and biofuel cells. Herein, we explored micro-diffusion behavior at different temperature gaps in microfluidic chip and enzyme-linked immunosorbent assay (ELISA) microplates. It exhibited that temperature gap could distinctly promote directional diffusion of molecules in microfluidic chip from high concentration zone to low concentration zone. In addition, experimental results of specially designed ELISA also partly confirmed that the temperature gap could effectively improve the performance of ELISA by 54.5%. In compared with conventional ELISA, the as-prepared temperature-gap functional module in microfluidic chip has obvious advantages of miniaturization, integration, customization, and low cost. All in all, the current work indicates that temperature-gap function has great potential in biomedical detection, food safety, and so on. It can be utilized to develop novel biological detection approaches and related instruments or microfluidic chips.
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This work was supported by Experimental Technology Special Fund of Shanghai Jiao Tong University (no. JCZXSJB2018-003), the National Key Scientific Research Project of China (nos. AWS15J006, SKLPBS1827 and 2017YFC1200900), Science and Technology Commission of Shanghai Municipality (no. 15441904800) and National Key Basic Research Program of China (973 Project) (no. 2017YFA0205300). It was carried out in Center for Advanced Electronic Materials and Devices (AEMD) of Shanghai Jiao Tong University.
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Zhi, X., Chen, L., Gao, S. et al. Temperature gap drives directed diffusion in microfluidic chip system. Microfluid Nanofluid 23, 40 (2019). https://doi.org/10.1007/s10404-019-2200-7
- Temperature gap
- Biological detection
- Microzone diffusion