How to improve the sensitivity of coplanar electrodes and micro channel design in electrical impedance flow cytometry: a study
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This paper describes a comprehensive analysis of the geometrical parameters influencing the sensitivity of a coplanar electrode layout for electrical impedance flow cytometry. The designs presented in this work have been simulated, fabricated, and tested. 3D finite element method was applied to simulate and improve the sensitivity of the coplanar designs for two spacings between electrodes. The proposed model uses conditional expressions to define spatially dependent material properties. The vertical and lateral sensitivities were evaluated for all the designs. The experimental results obtained with polystyrene beads show good agreement with the simulations. Precentering particles with dielectrophoresis allowed to control their position in the microchannel. The optimized designs are envisioned to be used for sizing and characterizing particles from single cells to cell aggregates.
KeywordsLab-on-a-Chip Impedance spectroscopy Flow cytometry Electrode design
The authors acknowledge the support from the CMi staff at EPFL for their technical assistance. The Ampere lab would like to acknowledge support from the Institut National de la Santé et de la Recherche Médicale (INSERM, Plan Cancer, Physicancer Program, Dynamo project). The authors also acknowledge the support of the Programme d’Avenir Lyon Saint-Etienne (PALSE mobility Grant) and the Laboratoire d’Excellence iMUST (ANR-10-LABX-0064/ANR-11-IDEX-0007) from University of Lyon as well as the Doctoral school 160 EEA of the University of Lyon for the mobility grants allocated.
Compliance with ethical standards
Conflict of interest
There are no conflicts to declare.
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