Inertial particle focusing dynamics in a trapezoidal straight microchannel: application to particle filtration

Research Paper
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Abstract

Inertial microfluidics has emerged recently as a promising tool for high-throughput manipulation of particles and cells for a wide range of flow cytometric tasks including cell separation/filtration, cell counting, and mechanical phenotyping. Inertial focusing is profoundly reliant on the cross-sectional shape of channel and its impacts on not only the shear field but also the wall-effect lift force near the wall region. In this study, particle focusing dynamics inside trapezoidal straight microchannels was first studied systematically for a broad range of channel Re number (20 < Re < 800). The altered axial velocity profile and consequently new shear force arrangement led to a cross-lateral movement of equilibration toward the longer side wall when the rectangular straight channel was changed to a trapezoid; however, the lateral focusing started to move backward toward the middle and the shorter side wall, depending on particle clogging ratio, channel aspect ratio, and slope of slanted wall, as the channel Reynolds number further increased (Re > 50). Remarkably, an almost complete transition of major focusing from the longer side wall to the shorter side wall was found for large-sized particles of clogging ratio K ~ 0.9 (K = a/Hmin) when Re increased noticeably to ~ 650. Finally, based on our findings, a trapezoidal straight channel along with a bifurcation was designed and applied for continuous filtration of a broad range of particle size (0.3 < K < 1) exiting through the longer wall outlet with ~ 99% efficiency (Re < 100).

Keywords

Inertial microfluidics Straight microchannel Trapezoidal Filtration Cell/particle sorting 

Notes

Acknowledgements

The first author would like to thank the SINGA scholarship sponsorship by A*STAR graduate academy, Singapore. This work was performed (in part) at the NSW and South Australian node of the Australian National Fabrication Facility under the National Collaborative Research Infrastructure Strategy to provide nano- and micro-fabrication facilities for Australia’s researchers. M.E.W. would like to acknowledge the support of the Australian Research Council through Discovery Project Grants (DP170103704 and DP180103003) and the National Health and Medical Research Council through the Careered Development Fellowship (APP1143377).

Supplementary material

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.School of Mechanical and Aerospace EngineeringNanyang Technological University (NTU)SingaporeSingapore
  2. 2.Bio-Manufacturing Programme, Singapore Institute of Manufacturing Technology (SIMTech)Agency for Science, Technology and Research (A*STAR)SingaporeSingapore
  3. 3.Stem Cell GroupBioprocessing Technology InstituteSingaporeSingapore
  4. 4.School of Biomedical EngineeringUniversity of Technology SydneySydneyAustralia
  5. 5.School of Medical and Health Sciences, Edith Cowan UniversityPerthAustralia
  6. 6.Institute of Molecular MedicineSechenov First Moscow State UniversityMoscowRussia

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