Capillary flow-driven blood plasma separation and on-chip analyte detection in microfluidic devices

Review
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Abstract

We report a comprehensive review on the capillary flow-driven blood plasma separation and on-chip analyte detection in microfluidic devices. Blood plasma separation is the primary sample preparation step prior to most biochemical assays. Conventionally, centrifugation is used for the sample preparation process. There are numerous works reporting blood plasma separation in microfluidic devices which aim at miniaturizing the sample preparation procedure. Capillary-based blood plasma separation shows promise in actualizing point-of-care diagnostic devices for applications in resource-limited settings including military camps and rural areas. In this review, the devices have been categorized based on active and passive plasma separation techniques used for the separation of plasma from capillary-driven blood sample. A comparison between different techniques used for blood plasma separation is outlined. On-chip detection of analytes present in the separated plasma obtained using some of these reported devices is also presented and discussed.

Keywords

Contact Angle PDMS Microfluidic Device Purification Efficiency PDMS Layer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

The authors would like to acknowledge SERB, DST, India (EMR/2014/001151), and IIT Madras (MEE1516843RFTPASHS) for the financial support.

References

  1. Abram E (2015) Screening and Diagnostic Tests. Medscape. http://emedicine.medscape.com/article/773832-overview. Accessed 20 Nov 2016
  2. AdvaMed (2012) Essentials of Diagnostics. DX Insights, pp 1–14Google Scholar
  3. Aran K, Fok A, Sasso LA, Kamdar N, Guan Y, Sun Q, Undar A, Zahn JD (2011) Microfiltration platform for continuous blood plasma protein extraction from whole blood during cardiac surgery. Lab Chip 11:2858–2868CrossRefGoogle Scholar
  4. Bhattacharya S, Datta A, Berg JM, Gangopadhyay S (2005) Studies on surface wettability of poly(dimethyl) siloxane (pdms) and glass under oxygen-plasma treatment and correlation with bond strength. J Microelectromech Syst 14(3):590–597CrossRefGoogle Scholar
  5. Bhattacharya S, Gao Y, Korampally V, Othman MT, Grant SA, Kleiboeker SB, Gangopadhyay K, Gangopadhyay S (2007) Optimization of design and fabrication processes for realization of a PDMS-SOG-silicon DNA amplification chip. J Microelectromech Syst 16(2):401–410CrossRefGoogle Scholar
  6. Bhattacharya S, Singh RK, Mandal S, Ghosh A, Bok S, Korampally V, Gangopadhyay K, Gangopadhyay S (2010) Plasma modification of polymer surfaces and their utility in building biomedical microdevices. J Adhes Sci Technol 24:2707–2739CrossRefGoogle Scholar
  7. Blattert C, Jurischka R, Schoth A, Kerth P, Menz W (2004) Separation of blood cells and plasma in microchannel bend structures. In: Lab-on-a-Chip: platforms, devices, and applications, vol 143, pp 143–151Google Scholar
  8. Chai J, Lu F, Li B, Kwok DY (2004) Wettability interpretation of oxygen plasma modified poly(methyl methacrylate). Langmuir 20:10919–10927CrossRefGoogle Scholar
  9. Chen C, Lin P, Chung C (2014) Microfluidic chip for plasma separation from undiluted human whole blood samples using low voltage contactless dielectrophoresis and capillary force. Lab Chip 14:1996CrossRefGoogle Scholar
  10. Coulson JM, Richardson JF, Backhurst JR, Harker JH (1991) Chemical engineering volume 2: particle technology and separation processes, 5th edn. Pergamo Press, OxfordGoogle Scholar
  11. Crowley TA, Pizziconi V (2005) Isolation of plasma from whole blood using planar microfilters for lab-on-a-chip applications. Lab Chip 5:922–929CrossRefGoogle Scholar
  12. Davis JA, Inglis DW, Morton KJ, Lawrence DA, Huang LR, Chou SY, Sturm JC, Austin RH (2006) Deterministic hydrodynamics: taking blood apart. PNAS 103:14779–14784CrossRefGoogle Scholar
  13. Dimov IK, Basabe-Desmonts L, Garcia-Cordero JL, Ross BM, Ricco AJ, Lee LP (2011) Stand-alone self-powered integrated microfluidic blood analysis system (SIMBAS). Lab Chip 11:845–850CrossRefGoogle Scholar
  14. Faivre M, Abkarian M, Bickraj K, Stone HA (2006) Geometrical focusing of cells in a microfluidic device: an approach to separate blood plasma. Biorheology 43:147–159Google Scholar
  15. Furlani EP (2007) Magnetophoretic separation of blood cells at the microscale. J Phys D Appl Phys 40:1313–1319CrossRefGoogle Scholar
  16. Ghubade A, Mandal S, Chaudhury R, Singh RK, Bhattacharya S (2009) Dielectrophoresis assisted concentration of micro-particles and their rapid quantitation based on optical means. Biomed Microdevices 11:987–995CrossRefGoogle Scholar
  17. Hosokawa K, Sato K, Ichikawa N, Maeda M (2004) Power-free poly(dimethylsiloxane) microfluidic devices for gold nanoparticle-based DNA analysis. Lab Chip 4(3):181–185CrossRefGoogle Scholar
  18. Hou HW, Bhagat AAS, Lee WC, Huang S, Han J, Lim CT (2011) Microfluidic devices for blood fractionation. Micro Mach 2:319–343Google Scholar
  19. Juncker D (2002) Capillary microfluidic systems for bio/chemistry. Ph.D. Thesis, Université de Neuchâtel, Anchorage, p 16Google Scholar
  20. Jung JY, Kwak HY (2007) Separation of microparticles and biological cells inside an evaporating droplet using dielectrophoresis. Anal Chem 79(13):5087–5092CrossRefGoogle Scholar
  21. Kar S, Maitia TK, Chakraborty S (2015) Capillarity-driven blood plasma separation on paper-based devices. Analyst 140:6473CrossRefGoogle Scholar
  22. Kersaudy-Kerhoas M, Sollier E (2013) Micro-scale blood plasma separation: from acoustophoresis to egg-beaters. Lab Chip 13:3323–3346CrossRefGoogle Scholar
  23. Khumpuang S, Tanaka T, Aita F, Meng Z, Ooe K, Ikeda M, Omori Y, Miyamura K, Yonezawa H, Matsumoto K, Sugiyama S (2007) Blood plasma separation device using capillary phenomenon. In: 14th International conference on solid-state sensors, actuators and microsystems, france, pp 1967–1970Google Scholar
  24. Kim YC, Kim S, Kim D, Park S, Park J (2010) Plasma extraction in a capillary-driven microfluidic device using surfactant-added poly(dimethylsiloxane). Sens Actuators B Chem 145(2):861–868MathSciNetCrossRefGoogle Scholar
  25. Kim Y, Kim K, Park Y (2012) Measurement techniques for red blood cell deformability: recent advances. In: Moschandreou TE (ed) Blood cell—an overview of studies in hematology. InTech, Rijeka, pp 167–194Google Scholar
  26. Kovarik ML, Gach PC, Ornoff DM, Wang Y, Balowski J, Farrag L, Allbritton NL (2012) Micro total analysis systems for cell biology and biochemical assays. Anal Chem 84(2):516–540CrossRefGoogle Scholar
  27. Kumar S, Bhushan P, Saha A, Bhattacharya S (2016) Diagnosis of communicable diseases using paper micro-fluidic platforms. In: Cheng C-M, Hsu M-Y, Wu MY-C (eds) Point-of-care diagnostics—new progresses and perspectives. IAPC Open Book and Monograph Platform (OBP), ZagrebGoogle Scholar
  28. Kuroda C, Ohki Y, Ashiba H, Fujimaki M, Awazu K, Tanaka T, Makishima M (2014) Microfluidic sedimentation system for separation of plasma from whole blood. In: IEEE sensors 2014 proceedings, Valencia, pp 1854–1857Google Scholar
  29. de Laplace PS, Bowditch N (1829) Méchanique céleste, Hillard, Gray, Little and Wilkins, BostonGoogle Scholar
  30. Lee KK, Ahn CH (2013) A new on-chip whole blood/plasma separator driven by asymmetric capillary forces. Lab Chip 13(16):3261–3267CrossRefGoogle Scholar
  31. Li X, Ballerini DR, Shen W (2012) A perspective on paper-based microfluidics: current status and future trends. Biomicrofluidics 6(1):011301–011313CrossRefGoogle Scholar
  32. Liana DD, Raguse B, Gooding JJ, Chow E (2012) Recent advances in paper-based sensors. Sensors 12:11505–11526CrossRefGoogle Scholar
  33. Madadi H, Casals-Terré J, Mohammadi M (2015) Self-driven filter-based blood plasma separator microfluidic chip for point-of-care testing. Biofabrication 7(2):025007CrossRefGoogle Scholar
  34. Manz A, Graber N, Widmer HM (1990) Miniaturized total chemical analysis systems: a novel concept for chemical sensing. Sens Actuators B 1(1–6):244–248. doi: 10.1016/0925-4005(90)80209-I CrossRefGoogle Scholar
  35. Maria MS, Kumar BS, Chandra TS, Sen AK (2015) Development of a microfluidic device for cell concentration and blood cell-plasma separation. Biomed Microdevices 17:115CrossRefGoogle Scholar
  36. Maria MS, Rakesh PE, Chandra TS, Sen AK (2016) Capillary flow based microfluidic device for blood plasma separation and glucose detection. Biomicrofluidics 10:054108CrossRefGoogle Scholar
  37. Maria MS, Rakesh PE, Chandra TS, Sen AK (2017) Capillary flow-driven microfluidic device with wettability gradient and sedimentation effects for blood plasma separation. Sci Rep 7:43457. doi: 10.1038/srep43457 CrossRefGoogle Scholar
  38. Mohammadi M, Madadi H, Casals-Terré J, Sellarès J (2015) Hydrodynamic and direct-current insulator-based dielectrophoresis (H-DC-iDEP) microfluidic blood plasma separation. Anal Bioanal Chem 407:4733–4744CrossRefGoogle Scholar
  39. Morgan H, Green NG (2003) AC electrokinetics: colloids and nanoparticles. Research Studies Press, BaldockGoogle Scholar
  40. Mukherjee S, Kang TG, Chen Y, Kim S (2009) Plasma separation from blood: the ‘lab-on-a-chip’ approach. Crit Rev Biomed Eng 37(6):517–529CrossRefGoogle Scholar
  41. Nakashima Y, Hata S, Yasuda T (2010) Blood plasma separation and extraction from a minute amount of blood using dielectrophoretic and capillary forces. Sens Actuators B 145:561–569CrossRefGoogle Scholar
  42. National Heart, Lung and Blood Institute (2012) Types of Blood Tests. US Department of Health and Human Services: National Institutes of Heath. https://www.nhlbi.nih.gov/health/health-topics/topics/bdt/types. Accessed 18 Nov 2016
  43. Noiphung J, Songjaroen T, Dungchai W, Henry CS, Chailapakul O, Laiwattanapaisal W (2013) Electrochemical detection of glucose from whole blood using paper-based microfluidic devices. Anal Chim Acta 788:39–45CrossRefGoogle Scholar
  44. Park S, Shabani R, Schumacher M, Kim Y, Bae Y, Lee K, Cho HJ (2016) On-chip whole blood plasma separator based on microfiltration, sedimentation and wetting contrast. Microsyst Technol 22:2077–2085CrossRefGoogle Scholar
  45. Pietrangelo A (2015) Enzyme Markers. Healthline. http://www.healthline.com/health/enzyme-markers#Risks4. Accessed 18 Nov 2016
  46. Sakamoto H, Hatsuda R, Miyamura K, Sugiyama S (2012) Plasma separation PMMA device driven by capillary force controlling surface wettability. Micro Nano Lett 7(1):64–67CrossRefGoogle Scholar
  47. Son JH, Lee SH, Hong S, Park S, Lee J, Dickey AM, Lee LP (2014) Hemolysis-free blood plasma separation Hemolysis-free blood plasma separation. Lab Chip 14:2287CrossRefGoogle Scholar
  48. Songjaroen T, Dungchai W, Chailapakul O, Henry CS, Laiwattanapaisal W (2012) Blood separation on microfluidic paper-based analytical devices. Lab Chip 12:3392–3398CrossRefGoogle Scholar
  49. Swiss Precision Diagnostics GmbH (1985) Clearblue Pregnancy Tests. Clearblue. http://uk.clearblue.com/. Accessed 10 Nov 2016
  50. Szydzik C, Khoshmanesh K, Mitchell A, Karnutsch C (2015) Microfluidic platform for separation and extraction of plasma from whole blood using dielectrophoresis. Biomicrofluidics 9:064120CrossRefGoogle Scholar
  51. Toner M, Irimia D (2005) Blood-on-a-chip. Annu Rev Biomed Eng 7:77–103CrossRefGoogle Scholar
  52. Tripathi S, Kumar YVBV, Prabhakar A, Joshi SS, Agrawal A (2015) Passive blood plasma separation at the microscale: a review of design principles and microdevices. J Micromech Microeng 25(8):083001CrossRefGoogle Scholar
  53. Whitesides GM (2006) The origins and the future of microfluidics. Nature 442:368–373. doi: 10.1038/nature05058 CrossRefGoogle Scholar
  54. Wu C, Hong L, Ou C (2012) Blood cell-free plasma separated blood samples with a cascading weir-type microfilter using dead-end Filtration. J Med Biol Eng 32(3):163–168CrossRefGoogle Scholar
  55. Yang X, Forouzan O, Brown TP, Shevkoplyas SS (2012) Integrated separation of blood plasma from whole blood for microfluidic paper-based analytical devices. Lab Chip 12:274CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2017

Authors and Affiliations

  1. 1.Department of Mechanical EngineeringIndian Institute of Technology MadrasChennaiIndia
  2. 2.Department of BiotechnologyIndian Institute of Technology MadrasChennaiIndia

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