Characterization and evaluation of 3D printed microfluidic chip for cell processing
Microfluidics has found ubiquitous presence in biological applications such as tissue spheroid fabrication and pharmacology investigation. The increasing prevalence and complexity demand a highly adaptable fabrication method for the rapid and convenient production of these microfluidic systems. 3D printing, as an emerging fabrication technique, was investigated in this paper. Microfluidic features were fabricated using two most widely used 3D printing technologies namely the inkjet printing and filament deposition techniques. The printing resolution, accuracy, repeatability, surface roughness, wetting ability, and biocompatibility of the printed microfluidic chips were characterized. The capability of 3D printing was demonstrated by printing a number of microfluidic devices such as rotational flow device and gradient generator. Results showed that 3D printing techniques were successful in making intricate microscale architectures and have the potential of greatly simplifying the manufacturing process.
Keywords3D printing Additive manufacturing Rapid prototyping Microfluidics Material characterization Cells Tissue engineering
Compliance with ethical standards
Conflict of interest
The authors do not have conflict of interest to declare.
- Chua C-K, Yeong W-Y, Leong K-F (2005) Rapid prototyping in tissue engineering: a state-of-the-art report. In: Virtual modelling and rapid manufacturing—advanced research in virtual and rapid prototyping, pp 19–27Google Scholar
- Takai H, Kojima M, Ohara K, Horade M, Tanikawa T, Mae Y, IEEE (2013) Microfluidic device for automated generation of toroidal-like spheroids. In: 2013 10th international conference on ubiquitous robots and ambient intelligence, pp 140–143Google Scholar
- Ota H, Kodama T, Miki N (2010a) Microfluidic experimental array using micro-rotation flow for producing size-controlled three-dimensional spheroids. Paper presented at the 2010 International Symposium on Micro-NanoMechatronics and Human Science (MHS), 7–10 Nov 2010Google Scholar
- Takai H, Kojima M, Ohara K, Horade M, Tanikawa T, Mae Y, Arai T (2013) Microfluidic device for automated generation of toroidal-like spheroids. Paper presented at the 2013 10th International Conference on Ubiquitous Robots and Ambient Intelligence (URAI), 30 Oct 2013–2 Nov 2013Google Scholar
- Yeong W-Y, Chua C-K, Leong K-F, Chandrasekaran M, Lee M-W (2005) Development of scaffolds for tissue engineering using a 3D inkjet model maker. In: Virtual modelling and rapid manufacturing-advanced research in virtual and rapid prototyping, pp 115–118Google Scholar
- Yeong WY, Yap CY, Mapar M, Chua CK (2014) State-of-the-art review on selective laser melting of ceramics. In: High value manufacturing: advanced research in virtual and rapid prototyping, pp 65–70. doi: 10.1201/b15961-14
- Zhang J, Yan S, Sluyter R, Li W, Alici G, Nguyen N-T (2014) Inertial particle separation by differential equilibrium positions in a symmetrical serpentine micro-channel. Sci Rep. doi: 10.1038/srep04527
- Zhu F, Macdonald NP, Cooper JM, Wlodkowic D (2013) Additive manufacturing of lab-on-a-chip devices: promises and challenges. In: Proceedings of SPIE-the international society for optical engineering, art. no. 892344. doi: 10.1117/12.2033400