Zebrafish has been used as an important vertebrate model of genetic screening and new drug development because of excellent characteristics, such as optical transparency, rapid ex vivo growth, and high genetic similarity to humans. Despite these advantages, studies on zebrafish are limited because of the lack of a robust and reliable method to manipulate zebrafish during microinjection and screening, as well as time-lapse imaging. In this work, a new microfluidic concept that utilizes a series of magnetically actuated artificial cilia integrated into a microchannel was employed to control the orientation of zebrafish larvae with a validated axial rotation capability. In contrast to conventional methods, the proposed method enables a highly accurate small-angle (0°–20°) stepwise axial rotation of a larva inside the microchannel with less detrimental effects on larval growth. The hemodynamics in a selected vessel was then imaged during the axial rotation of the tested larva to assist cardiovascular assessment. In addition, the bioactivity of the tested larvae remains stable without short-term negative effects after the imaging. The proposed platform, along with the provided analytical paradigm, can facilitate future zebrafish screening via microfluidics in the pharmaceutical industry.
Orientation control Zebrafish Artificial cilia Microfluidics
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This study was supported by Ministry of Science and Technology of Taiwan under Contracts Nos. MOST 104-2221-E-006-169 and MOST 102-2221-E-006-297-MY3 (to Chia-Yuan Chen). This work would not be possible without the facility provided by Center for Micro/Nano Science and Technology, National Cheng Kung University. The research was in part supported by the Ministry of Education, Taiwan, R.O.C., through the Aim for the Top University Project to National Cheng Kung University (NCKU).
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