Lab-on-Chip for testing myelotoxic effect of drugs and chemicals

Abstract

In the last 20 years, one of the main goals in the drug discovery field has been the development of reliable in vitro models. In particular, in 2006 the European Centre for the Validation of Alternative Methods has approved the colony-forming unit granulocytes–macrophages test, which is the first and currently unique test applied to evaluate the myelotoxicity of xenobiotics in vitro. The present work aimed at miniaturizing this in vitro assay by developing and validating a Lab-on-Chip platform consisting of a high number of bioreactor chambers with screening capabilities in a high-throughput regime.

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Acknowledgments

This work was financially supported by Fondazione Cariplo, Grant No. 2011-0322.

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Correspondence to Marco Rasponi.

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Rasponi, M., Gazaneo, A., Bonomi, A. et al. Lab-on-Chip for testing myelotoxic effect of drugs and chemicals. Microfluid Nanofluid 19, 935–940 (2015). https://doi.org/10.1007/s10404-015-1622-0

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Keywords

  • Lab-on-Chip
  • Microfluidics
  • Clonogenic test
  • CFU-GM
  • CB-MNC