Lab-on-Chip for testing myelotoxic effect of drugs and chemicals


In the last 20 years, one of the main goals in the drug discovery field has been the development of reliable in vitro models. In particular, in 2006 the European Centre for the Validation of Alternative Methods has approved the colony-forming unit granulocytes–macrophages test, which is the first and currently unique test applied to evaluate the myelotoxicity of xenobiotics in vitro. The present work aimed at miniaturizing this in vitro assay by developing and validating a Lab-on-Chip platform consisting of a high number of bioreactor chambers with screening capabilities in a high-throughput regime.

This is a preview of subscription content, access via your institution.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6


  1. Boorman G, Luster M, Dean J, Campbell M (1982) Assessment of myelotoxicity caused by environmental chemicals. Environ Health Perspect 43:129

    Article  Google Scholar 

  2. Gad SC (1990) Recent developments in replacing, reducing, and refining animal use in toxicologic research and testing. Toxicol Sci 15:8–16

    Article  Google Scholar 

  3. Hartung T, Daston G (2009) Are in vitro tests suitable for regulatory use? Toxicol Sci 111:233–237

    Article  Google Scholar 

  4. Jeon NL, Baskaran H, Dertinger SK, Whitesides GM, Van De Water L, Toner M (2002) Neutrophil chemotaxis in linear and complex gradients of interleukin-8 formed in a microfabricated device. Nat Biotechnol 20:826–830

    Article  Google Scholar 

  5. Kim C, Lee K, Kim JH, Shin KS, Lee K-J, Kim TS, Kang JY (2008) A serial dilution microfluidic device using a ladder network generating logarithmic or linear concentrations. Lab Chip 8:473–479

    Article  Google Scholar 

  6. Lee K et al (2009) Generalized serial dilution module for monotonic and arbitrary microfluidic gradient generators. Lab Chip 9:709–717

    Article  Google Scholar 

  7. Occhetta P et al (2015a) High-throughput microfluidic platform for adherent single cells non-viral gene delivery. RSC Adv 5:5087–5095

    Article  Google Scholar 

  8. Occhetta P et al (2015b) High-throughput microfluidic platform for 3D cultures of mesenchymal stem cells, towards engineering developmental processes. Sci Rep 5:10288

    Article  Google Scholar 

  9. Pessina A et al (2001) Prevalidation of a model for predicting acute neutropenia by colony forming unit granulocyte/macrophage (CFU-GM) assay. Toxicol In Vitro 15:729–740

    Article  Google Scholar 

  10. Pessina A et al (2004) A methylcellulose microculture assay for the in vitro assessment of drug toxicity on granulocyte/macrophage progenitors (CFU-GM). Altern Lab Anim ATLA 32:17–23

    Google Scholar 

Download references


This work was financially supported by Fondazione Cariplo, Grant No. 2011-0322.

Author information



Corresponding author

Correspondence to Marco Rasponi.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOCX 971 kb)

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Rasponi, M., Gazaneo, A., Bonomi, A. et al. Lab-on-Chip for testing myelotoxic effect of drugs and chemicals. Microfluid Nanofluid 19, 935–940 (2015).

Download citation


  • Lab-on-Chip
  • Microfluidics
  • Clonogenic test
  • CFU-GM
  • CB-MNC