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Microfluidics and Nanofluidics

, Volume 18, Issue 5–6, pp 955–966 | Cite as

A microfluidic method for the selection of undifferentiated human embryonic stem cells and in situ analysis

  • E. Jabart
  • S. Rangarajan
  • C. Lieu
  • J. Hack
  • I. Conboy
  • L. L. SohnEmail author
Research Paper

Abstract

Conventional cell-sorting methods such as fluorescence-activated cell sorting (FACS) or magnetic-activated cell sorting (MACS) can suffer from certain shortcomings such as lengthy sample preparation time, cell modification through antibody labeling, and cell damage due to exposure to high shear forces or to attachment of superparamagnetic Microbeads. In light of these drawbacks, we have recently developed a label-free, microfluidic platform that can not only select cells with minimal sample preparation but also enable analysis of cells in situ. We demonstrate the utility of our platform by successfully isolating undifferentiated human embryonic stem cells (hESCs) from a heterogeneous population based on the undifferentiated stem-cell marker SSEA-4. Importantly, we show that, in contrast to MACS or FACS, cells isolated by our method have very high viability (~90 %). Overall, our platform technology could likely be applied to other cell types beyond hESCs and to a variety of heterogeneous cell populations in order to select and analyze cells of interest.

Keywords

Human embryonic stem cells (hESCs) Cell sorting Label-free In situ analysis 

Notes

Acknowledgments

We would like to thank the Siebel Scholars Foundation for their support of E. J. and the W. M. Keck Foundation, California Institute for Regenerative Medicine CIRM RN1-0032, and NIH/NIA AG 027252 for partial support of this work. We thank M. Mir, K. Balakrishnan, W. Cousin, and C. Elabd for a critical reading of this manuscript and helpful comments.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • E. Jabart
    • 1
  • S. Rangarajan
    • 1
  • C. Lieu
    • 2
  • J. Hack
    • 3
  • I. Conboy
    • 1
  • L. L. Sohn
    • 3
    Email author
  1. 1.Department of BioengineeringUniversity of CaliforniaBerkeleyUSA
  2. 2.School of MedicineCreighton UniversityOmahaUSA
  3. 3.Department of Mechanical EngineeringUniversity of CaliforniaBerkeleyUSA

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