Microfluidics and Nanofluidics

, Volume 17, Issue 4, pp 745–750 | Cite as

Syringe-assisted point-of-care micropumping utilizing the gas permeability of polydimethylsiloxane

Research Paper

Abstract

By utilizing the high gas permeability of polydimethylsiloxane (PDMS), a simple syringe-assisted pumping method was introduced. A dead-end microfluidic channel was partially surrounded by an embedded microchamber, with a thin PDMS wall isolating the dead-end channel and the embedded microchamber. A syringe was connected with the microchamber port by a short tube, and the syringe plunger was manually pulled out to generate low pressure inside the microchamber. When sample liquid was loaded in the inlet port, air trapped in the dead-end channel would diffuse into the surrounding microchamber through the PDMS wall, creating an instantaneous pumping of the liquid inside the dead-end channel. By only pulling the syringe manually, a constant low flow with a rate ranging from 0.089 to 4 nl/s was realized as functions of two key parameters: the PDMS wall thickness and the overlap area between the dead-end channel and the surrounded microchamber. This method enabled point-of-care pumping without pre-evacuating the PDMS devices in a bulky vacuum chamber.

Keywords

Point-of-care Polydimethylsiloxane (PDMS) Pump 

Supplementary material

10404_2014_1356_MOESM1_ESM.pdf (5 kb)
Supplementary material 1 (PDF 5 kb)

Supplementary material 2 (WMV 1822 kb)

Supplementary material 3 (WMV 8073 kb)

Supplementary material 4 (WMV 3862 kb)

Supplementary material 5 (WMV 2197 kb)

References

  1. Dimov IK, Basabe-Desmonts L, Garcia-Cordero JL, Ross BM, Ricco AJ, Lee LP (2011) Stand-alone self-powered integrated microfluidic blood analysis system (SIMBAS). Lab Chip 11(5):845–850CrossRefGoogle Scholar
  2. Gervais L, Delamarche E (2009) Toward one-step point-of-care immunodiagnostics using capillary-driven microfluidics and PDMS substrates. Lab Chip 9(23):3330–3337CrossRefGoogle Scholar
  3. Hosokawa K, Sato K, Ichikawa N, Maeda M (2004) Power-free poly(dimethylsiloxane) microfluidic devices for gold nanoparticle-based DNA analysis. Lab Chip 4(3):181–185CrossRefGoogle Scholar
  4. Hosokawa K, Omata M, Sato K, Maeda M (2006) Power-free sequential injection for microchip immunoassay toward point-of-care testing. Lab Chip 6(2):236–241CrossRefGoogle Scholar
  5. Li W, Chen T, Chen Z, Fei P, Yu Z, Pang Y, Huang Y (2012) Squeeze-chip: a finger-controlled microfluidic flow network device and its application to biochemical assays. Lab Chip 12(9):1587–1590CrossRefGoogle Scholar
  6. Liang DY, Tentori AM, Dimov IK, Lee LP (2011) Systematic characterization of degas-driven flow for poly(dimethylsiloxane) microfluidic devices. Biomicrofluidics 5(2):024108–024116CrossRefGoogle Scholar
  7. Mark AE, Bruce KG (2006) A PDMS-based gas permeation pump for on-chip fluid handling in microfluidic devices. J Micromech Microeng 16(11):2396CrossRefGoogle Scholar
  8. Merkel TC, Bondar VI, Nagai K, Freeman BD, Pinnau I (2000) Gas sorption, diffusion, and permeation in poly(dimethylsiloxane). J Polym Sci, Part B: Polym Phys 38(3):415–434CrossRefGoogle Scholar
  9. Nilghaz A, Wicaksono DHB, Gustiono D, Abdul Majid FA, Supriyanto E, Abdul Kadir MR (2012) Flexible microfluidic cloth-based analytical devices using a low-cost wax patterning technique. Lab Chip 12(1):209–218CrossRefGoogle Scholar
  10. Ong W-L, Tang K-C, Agarwal A, Nagarajan R, Luo L-W, Yobas L (2007) Microfluidic integration of substantially round glass capillaries for lateral patch clamping on chip. Lab Chip 7(10):1357–1366CrossRefGoogle Scholar
  11. Sung JH, Kam C, Shuler ML (2010) A microfluidic device for a pharmacokinetic-pharmacodynamic (PK-PD) model on a chip. Lab Chip 10(4):446–455CrossRefGoogle Scholar
  12. Tang X, Zheng B (2011) A PDMS viscometer for assaying endoglucanase activity. Analyst 136(6):1222–1226MathSciNetCrossRefGoogle Scholar
  13. Trung NB, Saito M, Takabayashi H, Viet PH, Tamiya E, Takamura Y (2010) Multi-chamber PCR chip with simple liquid introduction utilizing the gas permeability of polydimethylsiloxane. Sens Actuators B Chem 149(1):284–290CrossRefGoogle Scholar
  14. Weibel DB, Siegel AC, Lee A, George AH, Whitesides GM (2007) Pumping fluids in microfluidic systems using the elastic deformation of poly(dimethylsiloxane). Lab Chip 7(12):1832–1836CrossRefGoogle Scholar
  15. Xia YN, Whitesides GM (1998) Soft lithography. Annu Rev Mater Sci 28:153–184CrossRefGoogle Scholar
  16. Yetisen AK, Akram MS, Lowe CR (2013) Paper-based microfluidic point-of-care diagnostic devices. Lab Chip 13(12):2210–2251CrossRefGoogle Scholar
  17. Ziegler J, Zimmermann M, Hunziker P, Delamarche E (2008) High-performance immunoassays based on through-stencil patterned antibodies and capillary systems. Anal Chem 80(5):1763–1769CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  1. 1.Sensors and MicroActuators Learning Laboratory (SMALL), Department of Electrical EngineeringUniversity at Buffalo, The State University of New York (SUNY at Buffalo)BuffaloUSA

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