Microfluidics and Nanofluidics

, Volume 14, Issue 5, pp 895–902

Microscale confinement features can affect biofilm formation

  • Aloke Kumar
  • David Karig
  • Rajesh Acharya
  • Suresh Neethirajan
  • Partha P. Mukherjee
  • Scott Retterer
  • Mitchel J. Doktycz
Short Communication

DOI: 10.1007/s10404-012-1120-6

Cite this article as:
Kumar, A., Karig, D., Acharya, R. et al. Microfluid Nanofluid (2013) 14: 895. doi:10.1007/s10404-012-1120-6

Abstract

The majority of bacteria in nature live in biofilms, where they are encased by extracellular polymeric substances (EPS) and adhere to various surfaces and interfaces. Investigating the process of biofilm formation is critical for advancing our understanding of microbes in their most common mode of living. Despite progress in characterizing the effect of various environmental factors on biofilm formation, work remains to be done in the realm of exploring the inter-relationship between hydrodynamics, microbial adhesion and biofilm growth. We investigate the impact of secondary flow structures, which are created due to semi-confined features in a microfluidic device, on biofilm formation of Shewanella oneidensis MR-1. Secondary flows are important in many natural and artificial systems, but few studies have investigated their role in biofilm formation. To direct secondary flows in the creeping flow regime, where the Reynolds number is low, we flow microbe-laden culture through microscale confinement features. We demonstrate that these confinement features can result in pronounced changes in biofilm dynamics as a function of the fluid flow rate.

Keywords

Microfluidics Biofilms Secondary flows Bacteria Micro-vortices 

Supplementary material

Supplementary Video 1: This video depicts the backward flow in the channel. The fluid is seeded with 500 nm fluorescent polystyrene particles. The higher magnification images clearly show the vortex structure (MPG 5378 kb)

10404_2012_1120_MOESM2_ESM.mpg (1.1 mb)
Supplementary Video 2: This video shows the time sequence of biofilm formation in the microfluidic device at 8 µL/hr. The subsequent frames are images that were taken 25 minutes apart. The video spans a total time for 20 hrs (MPG 1114 kb)
10404_2012_1120_MOESM3_ESM.pdf (1.6 mb)
Supplementary material 3 (PDF 1661 kb)

Copyright information

© Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • Aloke Kumar
    • 1
  • David Karig
    • 2
  • Rajesh Acharya
    • 1
  • Suresh Neethirajan
    • 3
  • Partha P. Mukherjee
    • 4
  • Scott Retterer
    • 1
  • Mitchel J. Doktycz
    • 1
  1. 1.Biosciences DivisionOak Ridge National LaboratoryOak RidgeUSA
  2. 2.Research and Exploratory Development DepartmentJohns Hopkins University Applied Physics LaboratoryBaltimoreUSA
  3. 3.School of EngineeringUniversity of GuelphGuelphCanada
  4. 4.Department of Mechanical EngineeringTexas A&M UniversityCollege StationUSA

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