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Clustering triple microbeads in a dynamic microarray for timing-controllable bead-based reactions

Microfluidics and Nanofluidics volume 14, pages 1039–1048 (2013)Cite this article

Abstract

In this study, we developed a dynamic microfluidic device that enables the clustering of three different types of microbeads in a trapping spot and the rearrangement of contacting modes of the clustered microbeads. To achieve these two functions, two features are added to the conventional dynamic microfluidic device. (1) To trap multiple beads, an extended trapping spot with sub-by-pass channels and a valve was employed. (2) To rearrange the clustered microbeads, trapping spots that work only by backward flow were added. The design of the microfluidic device was realized by calculations based on fluidic resistance. By using the designed device, we successfully clustered different types of hydrogel microbeads including target materials, and observed reactions between clustered microbeads. In addition, by rearranging the contacting modes of the clustered microbeads, the reaction could be initiated/terminated at the desired time. We found that this dynamic microfluidic device is applicable to the quantitative analysis of chemical reactions between small amounts of multiple materials.

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Acknowledgments

We thank Yuya Morimoto at the University of Tokyo for the helpful advice on preparing monodisperse hydrogel beads. T. Tonooka and T. Teshima are supported by Research Fellowships of the Japan Society for the Promotion of Science (JSPS) for Young Scientists, Japan.

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Authors and Affiliations

  1. Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo, 153-8505, Japan

    Taishi Tonooka, Tetsuhiko Teshima & Shoji Takeuchi

  2. Exploratory Research for Advanced Technology (ERATO), Japan Science and Technology Agency (JST), 4-6-1 Komaba, Meguro-ku, Tokyo, 153-8505, Japan

    Shoji Takeuchi

Authors
  1. Taishi Tonooka
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  2. Tetsuhiko Teshima
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  3. Shoji Takeuchi
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Correspondence to Shoji Takeuchi.

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Tonooka, T., Teshima, T. & Takeuchi, S. Clustering triple microbeads in a dynamic microarray for timing-controllable bead-based reactions. Microfluid Nanofluid 14, 1039–1048 (2013). https://doi.org/10.1007/s10404-012-1111-7

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  • DOI: https://doi.org/10.1007/s10404-012-1111-7

Keywords

  • Dynamic microarray
  • Microbeads handling
  • Microfluidics
  • Microvalve
  • Lab-on-a-chip