Rotationally controlled magneto-hydrodynamic particle handling for bead-based microfluidic assays

Abstract

This work presents a novel magnetic actuation scheme for advanced particle handling on our previously introduced, centrifugal microfluidic platform for array-based analysis of individual cells and beads. The conceptually simple actuation is based on the reciprocating motion of an elastomeric membrane featuring an integrated permanent magnet and a stationary magnet aligned along the orbit of a disc-based chamber. This compression chamber is placed at the downstream end of the particle capture chamber to induce centripetally directed, hydrodynamic lift forces on particles trapped in V-shaped geometrical barriers. Towards high frequencies of rotation, the on-disc magnet ceases to follow the rapidly oscillating magnetic field, so that the magnetic actuator is disabled during the initial, sedimentation-based filling of the trap array. At reduced spin speeds, the residence time of the magnetic actuator is sufficient to displace the magnetic actuator, resulting in a flow through the V-cup array that re-distributes, and eventually fully depletes, the previously trapped beads from the array. The same magnetic deflection scheme is also demonstrated to accelerate mixing, e.g. for upstream sample preparation.