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A developed competitive immunoassay based on impedance measurements for methamphetamine detection

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Abstract

In this study, an electro-microchip was successfully used to detect the impedance signals of various methamphetamine (MET) concentrations based on the developed competitive immunoassay method. MET is a commonly used drug often abused by drug addicts and can cause irregular behavior; therefore, MET concentration detection is important for quantitative analysis. In this study, gold nanoparticles (AuNPs) were introduced into an electro-microchip through the specific binding of antibodies, thus constructing a bridge between two electrodes and allowing electrons to move between them. The decreasing impedance value can be easily measured with a commercial LCR meter. According to the collected measurements, a significant difference was observed in impedance signals after 13 min when MET concentrations were reduced. Additionally, a clear, distinguished impedance (a steep slope for both impedance and MET concentration) in the frequency effect (100 Hz–1 MHz) was observed at 100 Hz. When the concentration of the anti-MET antibody–colloidal gold conjugates was diluted 100×, the detectable limit for MET concentration was 1 ng/mL with 0.5 μg/mL of BSA–MET conjugate. Therefore, the developed electro-microchip is advantageous because it is effective with small sample volumes (30 μL), is a form of rapid quantitative measurement, and works with smaller detectable concentrations than other existing commercial detection products which have the best limit of detection of 100 ng/mL.

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References

  • Alfonta L, Bardea A, Khersonsky O, Katz E, Willner I (2001) Chronopotentiometry and Faradaic impedance spectroscopy as signal transduction methods for the biocatalytic precipitation of an insoluble product on electrode supports: routes for enzyme sensors, immunosensors and DNA sensors. Biosens Bioelectron 16:675–687

    Article  Google Scholar 

  • Bronstein I, Voyta JC, Thorpe GHG, Kricka LJ, Armstrong G (1989) Chemiluminescent assay of alkaline phosphatase applied in an ultrasensitive enzyme immunoassay of thyrotropin. Clin Chem 35:1441–1446

    Google Scholar 

  • Bruchez M, Moronne M, Gin P, Weiss S, Alivisatos AP (1998) Semiconductor nanocrystals as fluorescent biological labels. Science 281:2013–2016

    Article  Google Scholar 

  • Castaneda MT, Merkoci A, Pumera M, Alegret S (2007) Electrochemical genosensors for biomedical applications based on gold nanoparticles. Biosens Bioelectron 22:1961–1967

    Article  Google Scholar 

  • Chan WCW, Nie SM (1998) Quantum dot bioconjugates for ultrasensitive nonisotopic detection. Science 281:2016–2018

    Article  Google Scholar 

  • Chen HJ, Kuo XS, Yang Z, Ni WH, Wang JF (2008) Shape- and size-dependent refractive index sensitivity of gold nanoparticles. Langmuir 24:5233–5237

    Article  Google Scholar 

  • Haab BB, Dunham MJ, Brown PO (2001) Protein microarrays for highly parallel detection and quantitation of specific proteins and antibodies in complex solutions. Genome Biol 2 research0004-1-13

  • Hage DS (1999) Immunoassays. Anal Chem 71:294–304

    Article  Google Scholar 

  • Ince R, Narayanaswamy R (2006) Analysis of the performance of interferometry, surface plasmon resonance and luminescence as biosensors and chemosensors. Anal Chim Acta 269:1–20

    Article  Google Scholar 

  • Ishikawa E, Hashida S, Kohno T, Hirota K (1991) Ultrasensitive enzyme immunoassay. Anal Sci 7:891–896

    Article  Google Scholar 

  • Karadeniz H, Erdem A, Caliskan A, Pereira CM, Pereira EM, Ribeiro JA (2007) Electrochemical sensing of silver tags labelled DNA immobilized onto disposable graphite electrodes. Electrochem Commun 9:2167–2173

    Article  Google Scholar 

  • Kim K, Kim NH, Park HK (2007) μAg particle-based molecular sensing/recognition via surface-enhanced Raman spectroscopy. Biosens Bioelectron 22:1000–1005

    Article  Google Scholar 

  • Lackie PM (1996) Immunogold silver staining for light microscopy. Histochem Cell Biol 106:9–17

    Article  Google Scholar 

  • Laureyn W, Nelis D, Gerwen PV, Baert K, Hermans L, Magnee R, Pireaux JJ, Maes G (2000) Nanoscaled interdigitated titanium electrodes for impedimetric biosensing. Sens Actuator B Chem 68:360–370

    Article  Google Scholar 

  • Li M, Lin YC, Su KC, Wang YT, Chang TC, Lin HP (2006) A novel real-time immunoassay utilizing an electro-immunosensing microchip and gold nanopatricles for signal enhancement. Sens Actuator B Chem 117:451–456

    Article  Google Scholar 

  • MacBeath G, Schreiber SL (2000) Printing proteins as microarrays for high-throughput function determination. Science 289:1760–1763

    Google Scholar 

  • Merkoci A, Aldavert M, Marin S, Alegret S (2005) New materials for electrochemical sensing V: nanoparticles for DNA labeling. Trac-Trends Anal Chem 24:341–349

    Article  Google Scholar 

  • Mezzasoma L, Hamilton TB, Cristina MD, Rossi R, Bistoni F, Crisanti A (2002) Antigen microarrays for serodiagnosis of infectious diseases. Clin Chem 48:121–130

    Google Scholar 

  • Nguyen B, Tanious FA, Wilson WD (2007) Biosensor-surface plasmon resonance: quantitative analysis of small molecule-nucleic acid interactions. Methods 42:150–161

    Article  Google Scholar 

  • Olson LG, Lo YS Jr, Beebe TP, Harris JM (2001) Characterization of silane-modified immobilized gold colloids as a substrate for surface-enhanced Raman spectroscopy. Anal Chem 73:4268–4276

    Article  Google Scholar 

  • Park SJ, Taton TA, Mirkin CA (2002) Array-based electrical detection of DNA with nanoparticle probes. Science 295:1503–1506

    Article  Google Scholar 

  • Porter R, van der Logt P, Howell S, Reay MK, Badley A (2001) An electro-active system of immuno-assay (EASI assay) utilizing self assembled monolayer modified electrodes. Biosens Bioelectron 16:875–885

    Article  Google Scholar 

  • Rao VS, Kripesh V, Yoon SW, Tay AAO (2006) A thick photoresist process for advanced wafer level packaging applications using JSR THB-151N negative tone UV Photoresist. J Micromech Microeng 16:1841–1846

    Article  Google Scholar 

  • Rossier JS, Girault HH (2001) Enzyme linked immunosorbent assay on a microchip with electrochemical detection. Lab Chip 1:153–157

    Article  Google Scholar 

  • Stuart DA, Haes AJ, Yonzon CR, Hicks EM, Van Duyne RP (2005) Biological applications of localised surface plasmonic phenomenae. IEE Proc Nanobiotechnol 152:13–32

    Article  Google Scholar 

  • Tam SW, Wiese R, Lee S, Gilmore J, Kumble KD (2002) Simultaneous analysis of eight human Th1/Th2 cytokines using microarrays. J Immunol Methods 261:157–165

    Article  Google Scholar 

  • Thaxton CS, Georganopoulou DG, Mirkin CA (2006) Gold nanoparticle probes for the detection of nucleic acid targets. Clin Chim Acta 363:120–126

    Article  Google Scholar 

  • Wang J (2003) Nanoparticle-based electrical DNA detection. Anal Chim Acta 500:247–257

    Article  Google Scholar 

  • Yakovleva J, Davidsson R, Lobanova A, Bengtsson M, Eremin S, Laurell T, Emnéus J (2002) Microfluidic enzyme immunoassay using silicon microchip with immobilized antibodies and chemiluminescence detection. Anal Chem 74:2994–3004

    Article  Google Scholar 

  • Yang LJ, Wang JM, Huang YL (2004a) The micro ion drag pump using indium-tin-oxide (ITO) electrodes to resist aging. Sens Actuator A Phys 111:118–122

    Article  Google Scholar 

  • Yang L, Li Y, Erf GF (2004b) Interdigitated array microelectrode-based electrochemical impedance immunosensor for detection of Escherichia coli O157:H7. Anal Chem 76:1107–1113

    Article  Google Scholar 

  • Yeh CH, Hung CY, Chang TC, Lin HP, Lin YC (2009a) An immunoassay using antibody–gold nanoparticle conjugate, silver enhancement and flatbed scanner. Microfluid Nanofluid 6:85–91

    Article  Google Scholar 

  • Yeh CH, Huang HH, Chang TC, Lin HP, Lin YC (2009b) Using an electro-microchip, a nanogold probe, and silver enhancement in an immunoassay. Biosens Bioelectron 24:1661–1666

    Article  Google Scholar 

  • Yeh CH, Chen WT, Chang TC, Lin HP, Lin YC (2009c) Development of an immunoassay based on impedance measurements utilizing an antibody–nanosilver probe, silver enhancement, and electro-microchip. Sens Actuator B Chem 139:387–393

    Article  Google Scholar 

  • Yeh CH, Chen WT, Lin HP, Chang TC, Lin YC (2010a) A newly developed immunoassay method based on optical measurement for protein A detection. Talanta 15:55–60

    Article  Google Scholar 

  • Yeh CH, Chang YH, Chang TC, Lin HP, Lin YC (2010b) Electro-microchip DNA-biosensor for bacteria detection. Analyst 135:2717–2722

    Article  Google Scholar 

  • Yoon CH, Cho JH, Oh HI, Kim MJ, Lee CW, Choi JW, Paek SH (2003) Development of a membrane strip immunosensor utilizing ruthenium as an electro-chemiluminescent signal generator. Biosens Bioelectron 19:289–296

    Article  Google Scholar 

  • Zhu H, Bilgin M, Bangham R, Hall D, Casamayor A, Bertone P, Lan N, Jansen R, Bidlingmaier S, Houfek T, Mitchell T, Miller P, Dean RA, Gerstein M, Snyder M (2001) Global analysis of protein activities using proteome chips. Science 293:2101–2105

    Article  Google Scholar 

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Acknowledgments

The authors would like to thank the Center for Micro/Nano Technology, National Cheng Kung University, Tainan, Taiwan, ROC for access to equipment and technical support. This research was partially supported by the Southern Taiwan Science Park Administration (STSPA), Taiwan, ROC under contract no. 99RC06 and 100CB02. The work was also partially supported by NCKU Top 100 University Advancement and the “Aim for the Top University Plan” of the National Chiao Tung University and Ministry of Education, Taiwan, ROC.

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Authors and Affiliations

  1. Department of Engineering Science, National Cheng Kung University, 1 University Road, Tainan, 701, Taiwan, ROC

    Chia-Hsien Yeh, Wei-Ting Wang & Yu-Cheng Lin

  2. Firstep Bioresearch, Inc., 3F, No. 2, Lane 31, Sec. 1, Huan-Tung Rd., Hsin-Shi, Tainan, 741, Taiwan

    Pi-Lan Shen

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  1. Chia-Hsien Yeh
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  2. Wei-Ting Wang
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Correspondence to Yu-Cheng Lin.

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Yeh, CH., Wang, WT., Shen, PL. et al. A developed competitive immunoassay based on impedance measurements for methamphetamine detection. Microfluid Nanofluid 13, 319–329 (2012). https://doi.org/10.1007/s10404-012-0964-0

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  • DOI: https://doi.org/10.1007/s10404-012-0964-0

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