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Microfluidics and Nanofluidics

, Volume 13, Issue 2, pp 273–278 | Cite as

Fractal-shaped microchannel design for a kinetic analysis of biochemical reaction in a delay line

  • Katsuki Hirata
  • Tetsuo Ichii
  • Hiroaki Suzuki
  • Tomoaki Matsuura
  • Kazufumi Hosoda
  • Tetsuya YomoEmail author
Research Paper

Abstract

Microfluidic systems have been widely used to investigate chemical and biochemical reactions in a small volume. Their geometries are designed to meet the time scale and detection scheme for specific reactions to be analyzed. However, the time scales of the reaction dynamics depend on the type of reactions and concentrations of reactants, and the required time resolution also varies even in a single reaction time course. A system design that is universally applicable to a wide range of time scales should save time and reduce costs. Here, we propose a microfluidic channel design in a fractal-shaped configuration for a kinetic analysis of biochemical reactions encapsulated in microdroplets. We demonstrate that this configuration is effective in resolving the time courses of β-glucuronidase-catalyzed hydrolysis reactions and in vitro translation/transcription reactions, which are different in their reaction time scales, by simply changing the levels of the target nodes in the fractal geometry.

Keywords

Fractal Droplet microfluidics Biochemical reaction Water-in-oil emulsion 

Notes

Acknowledgments

We thank Dr. Yasuaki Kazuta, Ms. Hitomi Komai, and Tomomi Sakamoto for producing the PURE system. This research was supported in part by “Special Coordination Funds for Promoting Science and Technology: Yuragi Project”, the Global COE (Centers of Excellence) Program of the Japanese Ministry of Education, Culture, Sports, Science and Technology.

Supplementary material

10404_2012_958_MOESM1_ESM.docx (509 kb)
Supplementary material 1 (DOCX 507 kb)

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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Katsuki Hirata
    • 1
  • Tetsuo Ichii
    • 2
  • Hiroaki Suzuki
    • 1
    • 2
  • Tomoaki Matsuura
    • 2
    • 3
  • Kazufumi Hosoda
    • 1
  • Tetsuya Yomo
    • 1
    • 2
    • 4
    Email author
  1. 1.Department of Bioinformatic Engineering, Graduate School of Information Science and TechnologyOsaka UniversityOsakaJapan
  2. 2.Exploratory Research for Advanced Technology (ERATO), Japan Science and Technology AgencyOsakaJapan
  3. 3.Department of Biotechnology, Graduate School of EngineeringOsaka UniversityOsakaJapan
  4. 4.Graduate School of Frontier BiosciencesOsaka UniversityOsakaJapan

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