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Cell motion and recovery in a two-stream microfluidic device

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Abstract

The motion of cells in a two-stream microfluidic device designed to extract cryoprotective agents from cell suspensions was tested under a range of conditions. Jurkat cells (lymphoblasts) in a 10% dimethylsulfoxide solution were driven in parallel with phosphate-buffered saline solution wash streams through single rectangular channel sections and multiple sections in series. The influence of cell-stream flow rate and cell volume fraction (CVF) on cell viability and recovery were examined. The channel depth was 500 μm, and average cell stream velocity within the channels was varied from 3.6 to 8.5 mm/s corresponding with cell stream Reynolds numbers of 2.6–6.0. Cell viability measured at device outlets was high for all cases examined indicating no significant cell damage within the device. Downstream of a single stage, cell recoveries measured 90–100% for average cell stream velocities ≥6 mm/s and for CVFs up to 20%. Cell recovery downstream of multistage devices also measured 90–100% after a critical device population time. This time was found to be five times the average cell residence time within the device. The measured recovery values were significantly larger than those typically obtained using conventional cell washing methods.

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References

  • Antonenas V, Bradstock K, Shaw P (2002) Effect of washing procedures on unrelated cord blood units for transplantation in children and adults. Cytotherapy 4:16

    Google Scholar 

  • Di Carlo D, Irimia D, Tompkins RG, Toner M (2007) Continuous inertial focusing, ordering, and separation of particles in microchannels. Proc Natl Acad Sci USA 104:18892–18897

    Article  Google Scholar 

  • Fahy GM (1986) The relevance of cryoprotectant “toxicity” to cryobiology. Cryobiology 23(1):1–13

    Article  MATH  Google Scholar 

  • Fleming KK, Longmire EK, Hubel A (2007) Numerical characterization of diffusion-based extraction in cell-laden flow through a microfluidic channel. J Biomech Eng 129:703–711

    Article  Google Scholar 

  • Glass K, Longmire EK, Hubel A (2008) Optimization of a microfluidic device for diffusion-based extraction of DMSO from a cell suspension. Int J Heat Mass Transfer 51(23):5749–5757

    Article  MATH  Google Scholar 

  • Gurtovenko AA, Anwar J (2007) Modulating the structure and properties of cell membranes: the molecular mechanism of action of dimethyl sulfoxide. J Phys Chem B 111:10453–10460

    Article  Google Scholar 

  • Hawkes JJ, Barber RW, Emerson DR, Coakley WT (2004) Continuous cell washing and mixing driven by an ultrasound standing wave within a microfluidic channel. Lab Chip 4:446–452

    Article  Google Scholar 

  • Kumar M, Felke D, Belovick J (2005) Fractionation of cell mixtures using acoustic and laminar flow fields. Biotechnol Bioeng 89:129–137

    Article  Google Scholar 

  • Mata C, Longmire E, McKenna D, Glass K, Hubel A (2008) Experimental study of diffusion based extraction from a cell suspension. Microfluid Nanofluid 5:529–540

    Article  Google Scholar 

  • Perotti CG, Fante CD, Viarengo G, Papa P, Rocchi L, Bergamaschi P, Bellotti L, Marchesi A, Salvaneschi L (2004) A new automated cell washer device for thawed cord blood units. Transfusion 44:900–906

    Article  Google Scholar 

  • Petersson F, Nilsson A, Jonsson H, Laurell T (2005) Carrier medium exchange through ultrasonic particle switching in microfluidic channels. Anal Chem 77:1216–1221

    Article  Google Scholar 

  • Sethu P, Moldawer LL, Mindrinos MN, Scumpia PO, Tannahill CL, Wilhelmy J, Efron PA, Brownstein BH, Tompkins RG, Toner M (2006a) Microfluidic isolation of leukocytes from whole blood for phenotype and gene expression analysis. Anal Chem 78:5453–5461

    Article  Google Scholar 

  • Sethu P, Sin A, Toner M (2006b) Microfluidic diffusive filter for apheresis (leukapheresis). Lab Chip 6:83–89

    Article  Google Scholar 

  • Yamada M, Kobayashi J, Yamato M, Seki M, Okano T (2008) Millisecond treatment of cells using microfluidic devices via two-step carrier-medium exchange. Lab Chip 8:772–778

    Article  Google Scholar 

  • Yang S, Undar A, Zahn JD (2005) Blood plasma separation in microfluidic channels using flow rate control. ASAIO J 51:585–590

    Article  Google Scholar 

Download references

Acknowledgments

This work was funded by the National Institutes of Health (R21EB004857). The authors thank Dave Hultman for assistance with facility design, Brian Darr for help with cell culturing and Jacob Hanna for help with additional experiments.

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Authors and Affiliations

  1. Aerospace Engineering and Mechanics, University of Minnesota, 110 Union Street SE, Minneapolis, MN, 55455, USA

    Clara Mata & Ellen Longmire

  2. Clinical Cell Therapy Laboratory, University of Minnesota, 420 Delaware Street, Minneapolis, MN, 55455, USA

    David McKenna

  3. Department of Mechanical Engineering, University of Minnesota, 1100 Mechanical Engineering, 111 Church Street SE, Minneapolis, MN, 55455, USA

    Katie Glass & Allison Hubel

Authors
  1. Clara Mata
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  2. Ellen Longmire
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  3. David McKenna
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  4. Katie Glass
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  5. Allison Hubel
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Corresponding author

Correspondence to Allison Hubel.

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Mata, C., Longmire, E., McKenna, D. et al. Cell motion and recovery in a two-stream microfluidic device. Microfluid Nanofluid 8, 457–465 (2010). https://doi.org/10.1007/s10404-009-0470-1

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  • DOI: https://doi.org/10.1007/s10404-009-0470-1

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