Various cell stimulation experiments have been traditionally conducted regarding observation of cells to specific stimulative factors, and in light of this area of study, we report a new method by utilizing micro-beads. HeLa cells and MC3T3 cells are cultured in straight PDMS (polydimethylsiloxane) microfluidic devices and are stimulated by sterilized polystyrene micro-beads. Cell culture medium either with or without micro-beads are introduced in microfluidic cell culturing chambers at a specific time interval, and stimulated cells are observed using an inverted microscope. The results show that cells exposed under micro-bead stimulation perform at a higher growth rate than those under normal conditions. This paper demonstrates that micro-beads can be used as a physical stimulation factor and affect cell growth behaviors.
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This work was supported by ICBIN of Seoul R&BD program (Grant no. 10816) and National Core Research Center (NCRC) for Nanomedical Technology of the Korea Science and Engineering Foundation (Grant no. R15-2004-024-01001-0).
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Kim, TJ., Kim, SJ. & Jung, HI. Physical stimulation of mammalian cells using micro-bead impact within a microfluidic environment to enhance growth rate. Microfluid Nanofluid 6, 131 (2009). https://doi.org/10.1007/s10404-008-0310-8
- HeLa cell
- MC3T3 cell
- Micro-bead stimulation
- Microfluidic channel