Digital microfluidic design and optimization of classic and new fluidic functions for lab on a chip systems
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This paper deals with microfluidic studies for lab-on-a-chip development. The first goal was to develop microsystems immediately usable by biologists for complex protocol integrations. All fluid operations are performed on nano-liter droplet independently handled solely by electrowetting on dielectric (EWOD) actuation. A bottom-up architecture was used for chip design due to the development and validation of elementary fluidic designs, which are then assembled. This approach speeds up development and industrialization while minimizing the effort in designing and simplifying chip-fluidic programming. Dispensing reproducibility for 64 nl droplets obtained a CV below 3% and mixing time was only a few seconds. Ease of the integration was demonstrated by performing on chip serial dilutions of 2.8-folds, four times. The second part of this paper concerns the development of new innovative fluidic functions in order to extend EWOD-actuated digital fluidics’ capabilities. Experiments of particle dispensing by EWOD droplet handling are reported. Finally, work is shown concerning the coupling of EWOD actuation and magnetic fields for magnetic bead manipulation.
KeywordsDigital microfluidic Droplet Electrowetting Lab on a chip
The authors would like to thank Olivier Constantin, Gaël Castellan and Nadine David for preparation of the fluidic components, and Nicolas Chiaruttini for experimentations with magnetic beads. This work was funded by the French research ministry.
- Bavière R, Fouillet Y (2006) Dynamics of droplet transport induced by electrowetting actuation. SHF-MicrofluidicsGoogle Scholar
- Berge B (1993) Electrocapillarity and wetting of insulator films by water. C R Acad Sci Serie Ii 317(2):157–163Google Scholar
- Fouillet Y, Jary D, Brachet AG, Chabrol C, Boutet J, Clementz P, Lauro D, Charles R, Peponnet C (2005) Design and validation of a complex generic fuidic microprocessor based on ewod droplet for biological applications. Micro Total Analysis Systems, Boston, pp 59–69Google Scholar
- Fouillet Y, Jary D, Brachet AG, Berthier J, Blervaque R, Davoust L, Roux JM, Achard JL, Peponnet C (2006) Ewod digital microfluidics for lab on a chip. ASME ICNMM2006, Limerick, IrelandGoogle Scholar
- Jary D, Chollat-Namy A, Fouillet Y, Boutet J, Chabrol C, Castellan G, Gasparutto D, Peponnet C (2006) DNA repair enzyme analysis on EWOD fluidic microprocessor. Nanotech2006, Boston, USAGoogle Scholar
- Oprins H, Nicole C, Baret JC, Van der Veken G, Lasance C, Baelmans M (2005) On-chip liquid cooling with integrated pump technology. Proceedings twenty-first Semitherm, San Jose, CAGoogle Scholar
- Ulrike Lehmann, Vandevyver C, Virendra K. Parashar, Martin AM Gijs (2006) Droplet-based DNA purification in a magnetic lab-on-a-chip. Angew Chem Int Ed 45(19):3062–3067Google Scholar