Abstract
Nanofluidic channels of ∼40×60 nm (width × depth) were fabricated with focused ion beam (FIB) milling instrument on a silicon nitride (Si3N4) film. Stained λ-phage DNA molecules were driven into these open channels by capillary force and observed with fluorescence microscopy. The movements of DNA molecule in these channels were discussed. These sub-100 nm scale channels may be useful in studying single biomacromolecules.
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Aksimentiev A, Heng JB, Timp G, Schulten K (2004) Biophys J 87:2086–2097
Deamer DW, Branton D (2002) Acc Chem Res 35:817–825
Fabrizio ED, Fillipo R, Cabrini S et al (2004) J Phys Condens Matter 16:S3517–S3535
Han J, Craighead HG (2000) Science 288:1026–1029
Ho C, Qiao R, Heng JB et al (2005) Proc Natl Acad Sci USA 102:10445–10450
Li J, Stein D, Mcmullan C et al (2001) Nature 412:166–169
Meller A, Nivon L, Branton D (2001) Phys Rev Lett 86:3435–3438
Nakane J, Akeson M, Marziali A (2002) Electrophoresis 23:2592–2601
Siwy Z, Dobrev D, Neumann R et al (2003) Appl Phys A 76:781–785
Storm AJ, Chen JH et al (2003) Nat Mater 2:537–540
Studer V, Pepin A, Chen Y, Ajdari A (2002) Microelectron Eng 61/62:915–920
Tegenfeldt JO, Prinz C, Han Cao et al (2004a) Anal Bioanal Chem 378:1678–1692
Tegenfeldt JO, Prinz C, Cao H et al (2004b) Proc Natl Acad Sci USA 101:10979–10983
Veerman JA, Otter AM, Kuipers L, Van Hulst NF (1998) Appl Phys Lett 24:3115–3117
Wang Kaige, Dou Shuoxing, Wang Pengye et al (2004) Physics 6:9–14
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Wang, KG., Yue, S., Wang, L. et al. Manipulating DNA molecules in nanofluidic channels. Microfluid Nanofluid 2, 85–88 (2006). https://doi.org/10.1007/s10404-005-0057-4
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DOI: https://doi.org/10.1007/s10404-005-0057-4