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Viability variation of T-cells under ultrasound exposure according to adhesion condition with bubbles

  • Original Article–physics & Engineering
  • Published:
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Abstract

Purpose

Although cellular immunotherapy is expected as a new cancer treatment, its therapeutic efficiency is limited in solid tumors, because most cells return to the bloodstream rather than adhere to the target site. Therefore, we are motivated to develop a technique to concentrate the cells in the blood flow using active control of bubble-surrounded cells under ultrasound exposure considering both aspects of cell controllability and viability.

Methods

We prepared a lipid bubble conjugating ligand to adhere to the surface of the T-cells. First, we evaluated the cell controllability by retaining the cells on a wall of an artificial blood vessel through continuous ultrasound exposure. Next, we investigated the cell viability under ultrasound exposure in a suspension with various bubble concentrations.

Results

We estimated the concentration of bubbles when the adhesion to the cell surface was saturated. Then, we evaluated the cell viability with various conditions of ultrasound exposure and bubble concentrations. However, it was confirmed that cell damage occurred under conditions that achieved proper control of the cells. Therefore, we exposed the cells to burst waves to reduce the applied ultrasound intensity. Consequently, the significant increase in cell viability was confirmed to be inversely proportional to the duty ratio.

Conclusion

To retain cells on a vessel wall, determining the appropriate ultrasound condition including sound pressure and waveform is important to maintain cell viability.

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Data availability

The authors confirm that the data supporting the findings of this study are available within the article.

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Acknowledgements

This research was supported by a grant from the Japan Society for the Promotion of Science (JSPS) through KAKENHI Grant No. 20H04547, and the Uehara Memorial Foundation.

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Authors and Affiliations

  1. Graduate School of BASE, Tokyo University of Agriculture and Technology, Koganei, Tokyo, 184-8588, Japan

    Naoya Kajita, Yoshiki Ito, Shunya Watanabe & Kohji Masuda

  2. National Center for Child Health and Development, Setagaya, Tokyo, 157-8535, Japan

    Yoshitaka Miyamoto

  3. Faculty of Pharma-Science, Teikyo University, Itabashi, Tokyo, 173-8605, Japan

    Daiki Omata, Kazuo Maruyama & Ryo Suzuki

  4. Advanced Comprehensive Research Organization (ACRO), Itabashi, Tokyo, 173-8605, Japan

    Kazuo Maruyama & Ryo Suzuki

Authors
  1. Naoya Kajita
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  2. Yoshiki Ito
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  3. Shunya Watanabe
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  4. Yoshitaka Miyamoto
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Correspondence to Kohji Masuda.

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Kajita, N., Ito, Y., Watanabe, S. et al. Viability variation of T-cells under ultrasound exposure according to adhesion condition with bubbles. J Med Ultrasonics 50, 121–129 (2023). https://doi.org/10.1007/s10396-022-01277-5

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  • DOI: https://doi.org/10.1007/s10396-022-01277-5

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