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New approach for local cancer treatment using pulsed high-intensity focused ultrasound and phase-change nanodroplets

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Abstract

Purpose

The aim of this study was to investigate the combination effects of pulsed HIFU (pHIFU) and phase-change nanodroplets (PCND) as a sensitizer on efficient induction of mechanical effects of pHIFU and chemically enhanced tumor growth inhibition for local anti-tumor therapy.

Method

Changes in growth of colon 26 tumor tissue inoculated onto CDF1 mice were evaluated by the following treatments. (1) pHIFU exposure (1.1 MHz, 3.2 kW/cm2, 300 cycles, and 50 ms interval) for 60 s, (2) PCND (1 %) injection, (3) adriamycin (4 mg/kg) injection, (4) pHIFU exposure after PCND injection, and (5) pHIFU exposure after PCND + adriamycin injection simultaneously.

Results

Significant changes in tumor growth were observed in the group with combination of pHIFU and PCND, although single therapy did not show any significant difference. PCND enhanced mechanical tissue fractionation by pHIFU, which was detectable by Real-time tissue elastography. Moreover, the combination of pHIFU and PCND + Adriamycin suppressed the tumor growth for 2 weeks, and 3 of 4 mice did not show any sign of regrowth during the 30-day observation.

Conclusion

The combination of pHIFU and PCND exerted a significant anti-tumor effect and may be a new candidate for treatment of locally advanced cancer.

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Acknowledgments

This work was supported in part by the Research and Development Committee Program of the Japan Society of Ultrasound in Medicine and Biology, and also by the Japan Society for the Promotion of Science (JSPS) through the “Funding Program for World-Leading Innovative R&D on Science and Technology (FIRST Program),” initiated by the Council for Science and Technology Policy (CSTP). The authors deeply thank Prof. Umemura of Tohoku University for discussion and encouragement.

Conflict of interest

Reiko Ashida, Ken-ichi Kawabata, Takashi Maruoka, Rei Asami, Hideki Yoshikawa, Rena Takakura, Tastuya Ioka, Kazuhiro Katayama, and Sachiko Tanaka declare that they have no conflict of interest.

Ethical consideration

All institutional and national guidelines for the care and use of laboratory animals were followed.

Author information

Authors and Affiliations

  1. Department of Cancer Survey and Gastrointestinal Oncology, Osaka Medical Center for Cancer and Cardiovascular Diseases, Osaka, Japan

    Reiko Ashida, Tatsuya Ioka & Kazuhiro Katayama

  2. Research & Development Group, Hitachi, Ltd., 1-280 Higashi Koigakubo, Kokubunji, Tokyo, 180-8601, Japan

    Ken-ichi Kawabata, Takashi Maruoka, Rei Asami & Hideki Yoshikawa

  3. Department of Cancer Prevention, Osaka Center for Cancer and Cardiovascular Diseases Prevention, Osaka, Japan

    Rena Takakura & Sachiko Tanaka

Authors
  1. Reiko Ashida
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  2. Ken-ichi Kawabata
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  3. Takashi Maruoka
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  4. Rei Asami
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  6. Rena Takakura
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  9. Sachiko Tanaka
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Corresponding author

Correspondence to Ken-ichi Kawabata.

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Ashida, R., Kawabata, Ki., Maruoka, T. et al. New approach for local cancer treatment using pulsed high-intensity focused ultrasound and phase-change nanodroplets. J Med Ultrasonics 42, 457–466 (2015). https://doi.org/10.1007/s10396-015-0634-4

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  • DOI: https://doi.org/10.1007/s10396-015-0634-4

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