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Participation of mast cells in angiogenesis in the border zone of myocardial infarction in rats

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Abstract

Background

We hypothesized that mast cells may participate in coronary angiogenesis in acute myocardial infarction, contributing to myocardial salvage.

Methods

The left coronary artery was occluded in control (n = 30) and Ws rats (n = 30), which genetically lacked c-kit, resulting in a mast cell deficiency. Four weeks later, the infarct area, i.e., infarct core and surrounding infarct areas, and the non-infarct area were assessed histopathologically. The mast cell and small vessel densities were assessed using toluidine blue and alkaline phosphatase staining. Myocardial perfusion was assessed by myocardial contrast echocardiography (MCE).

Results

In Ws rats, the percentage infarct core area increased (p < 0.05) compared with the controls, whereas the percentage surrounding infarct area decreased (p < 0.01). Mast cell density increased most in the surrounding infarct area (p < 0.01) in control rats, whereas mast cells were absent in Ws rats. Compared with the controls, coronary microvessel density decreased in the surrounding infarct area in Ws rats (p < 0.01). MCE showed that the percentage infarct core area, i.e., perfusion defect, increased (p < 0.05) and the percentage surrounding infarct area, i.e., reduced perfusion area, decreased (p < 0.01) in Ws rats.

Conclusion

Mast cells may participate in promoting coronary angiogenesis in the infarct area surrounding the infarct core, contributing to attenuation of left ventricular dysfunction.

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Authors and Affiliations

  1. Department of Cardiology and Hematology, Fukushima Medical University, Hikarigaoka 1, Fukushima, 960-1295, Japan

    Takayoshi Yamaki, Masumi Iwai-Takano, Hiroyuki Yaoita, Kazuei Ogawa, Hiroko Tajima, Yasuchika Takeishi & Yukio Maruyama

Authors
  1. Takayoshi Yamaki
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  2. Masumi Iwai-Takano
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  3. Hiroyuki Yaoita
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  4. Kazuei Ogawa
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  5. Hiroko Tajima
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  6. Yasuchika Takeishi
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  7. Yukio Maruyama
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Corresponding author

Correspondence to Masumi Iwai-Takano.

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Yamaki, T., Iwai-Takano, M., Yaoita, H. et al. Participation of mast cells in angiogenesis in the border zone of myocardial infarction in rats. J Med Ultrasonics 36, 119–127 (2009). https://doi.org/10.1007/s10396-009-0229-z

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  • DOI: https://doi.org/10.1007/s10396-009-0229-z

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