Purpose
Ultrasonically induced cell damage and active oxygen generation with photofrin II were compared using the same in vitro insonation setup.
Methods
Sarcoma 180 cells suspended in an air-saturated phosphate-buffered saline solution were exposed to ultrasound at 2 MHz for not more than 60 s in the presence and absence of photofrin II. The trypan blue exclusion test was used to determine viability. Lipid peroxidation in cell membranes was estimated by measuring the quantity of reactive substance produced from thiobarbituric acid added immediately after the cells had been exposured to ultrasound.
Results
Significant enhancement of the rates of ultrasonically induced cell damage and lipid peroxidation was demonstrated with photofrin II (20–80 µg/ml), and the two rates were closely associated. Enhancement of both rates by photofrin II was suppressed by 10 mM of histidine.
Conclusion
These results suggest that ultrasonically generated active oxygen is a primary factor in ultrasonically induced cell damage in the presence of photofrin II.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Author information
Authors and Affiliations
Corresponding author
About this article
Cite this article
Yumita, N., Umemura, Si. Ultrasonically induced cell damage and membrane lipid peroxidation by photofrin II: mechanism of sonodynamic activation. J Med Ultrasonics 31, 35–40 (2004). https://doi.org/10.1007/s10396-003-0003-6
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s10396-003-0003-6