, 3:171 | Cite as

Survivorship in Wild Frogs Infected with Chytridiomycosis



Chytridiomycosis is an emerging infectious disease that has been implicated as the causative agent of many recent amphibian population declines and extinctions that have taken place in relatively pristine locations worldwide. While there exists a growing body of literature regarding the effect of the fungus on experimentally infected frogs, few studies have examined the effect of the fungus on apparently healthy wild frogs from nondeclining, infected populations. We examined the temporal pattern of chytrid infection in individually marked Stony Creek Frogs (Litoria wilcoxii) at a lowland site in southeast Queensland, Australia. We provide the first evidence that wild frogs are capable of both acquiring chytridiomycosis as adults, and also of clearing their infections entirely. Changes in disease status in individual frogs largely tracked changing climatic conditions, with infections tending to appear in cooler months and disappearing in warmer months. Though 27.2% of the adult frogs we sampled were infected at some point in the study, we found no evidence that chytridiomycosis was negatively affecting adult survivorship, suggesting either: (1) chytrid-induced mortality in this population is generally restricted to metamorphs and juveniles; (2) this population was not exposed to conditions which favored lethal disease outbreaks; or (3) this population has evolved sufficient resistance to the disease to persist relatively unaffected.


Batrachochytrium dendrobatidis amphibian declines chytridiomycosis survivorship chytrid Litoria wilcoxii 


  1. Berger L, Speare R, Daszak P, Green DE, Cunningham AA, Goggin CL, et al. (1998) Chytridiomycosis causes amphibian mortality associated with population declines in the rain forests of Australia and Central America. Proceedings of the National Academy of Science, USA 95:9031–9036CrossRefGoogle Scholar
  2. Berger L, Speare R, Hyatt A (1999) Chytrid fungi and amphibian declines: overview, implications and future directions. In: Campbell A (editor), Declines and Disappearances of Australian Frogs, Canberra, Australia: Environment Australia, pp 23–33Google Scholar
  3. Berger L, Speare R, Hines HB, Marantelli G, Hyatt AD, McDonald KR, et al. (2004) Effect of season and temperature on mortality in amphibians due to chytridiomycosis. Australian Veterinary Journal 82:31–36Google Scholar
  4. Blaustein AR, Kiesecker JM (2002) Complexity in conservation: lessons from the global decline of amphibian populations. Ecology Letters 5:597–608CrossRefGoogle Scholar
  5. Bosch J, Martínez-Solano I, García-París M (2001) Evidence of a chytrid fungus infection involved in the decline of the common midwife toad (Alytes obstetricans) in protected areas of central Spain. Biological Conservation 97:331–337CrossRefGoogle Scholar
  6. Boyle DG, Boyle DB, Olsen V, Morgan JAT, Hyatt AD (2004) Rapid quantitative detection of chytridiomycosis (Batrachochytrium dendrobatidis) in amphibian samples using real-time Taqman PCR assay. Diseases of Aquatic Organisms 60:141–148Google Scholar
  7. Bradley GA, Rosen PC, Sredl MJ, Jones TR, Longcore JE (2002) Chytridiomycosis in native Arizona frogs. Journal of Wildlife Diseases 38:206–212Google Scholar
  8. Briggs C, Vredenburg VT, Knapp R, Rachowicz LJ (2005) Investigating the population-level effects of chytridiomycosis: an emerging infectious disease of amphibians. Ecology 86:3149–3159CrossRefGoogle Scholar
  9. Carey C (2000) Infectious disease and worldwide declines of amphibian populations, with comments on emerging diseases in coral reef organisms and in humans. Environment Health Perspective 108:143–150CrossRefGoogle Scholar
  10. Collins JP, Storfer A (2003) Global amphibian declines: sorting the hypotheses. Diversity and Distributions 9:89–98CrossRefGoogle Scholar
  11. Daszak P, Berger L, Cunningham AA, Hyatt AD, Green DE, Speare R (1999) Emerging infectious diseases and amphibian population declines. Emerging Infectious Diseases 5:735–748CrossRefGoogle Scholar
  12. Daszak P, Cunningham AA, Hyatt AD (2003) Infectious disease and amphibian population declines. Diversity and Distributions 9:141–150CrossRefGoogle Scholar
  13. Daszak P, Strieby A, Cunningham AA, Longcore JE, Brown CC, Porter D (2004) Experimental evidence that the bullfrog (Rana catesbeiana) is a potential carrier of chytridiomycosis, an emerging fungal disease of amphibians. Herpetological Journal 14:201–207Google Scholar
  14. Davidson C, Shaffer HB, Jennings MR (2001) Declines of the California red-legged frog: climate, UV-B, habitat, and pesticides hypotheses. Ecological Applications 11:464–479Google Scholar
  15. Davidson EW, Parris M, Collins JP, Longcore JE, Pessier AP, Brunner J (2003) Pathogenicity and transmission of chytridiomycosis in tiger salamanders (Ambystoma tigrinum). Copeia 601–607Google Scholar
  16. Donnellan SC, Mahony MJ (2004) Allozyme, chromosomal and morphological variability in the Litoria lesueuri species group (Anura: Hylidae), including description of a new species. Australian Journal of Zoology 52:1–28CrossRefGoogle Scholar
  17. Gillespie GR (2001) The role of introduced trout in the decline of the spotted tree frog (Litoria spenceri) in south-eastern Australia. Biological Conservation 100:187–198CrossRefGoogle Scholar
  18. Gillespie G, Hines H (1999) Status of temperate riverine frogs in south-eastern Australia. In: Campbell A (editor), Declines and Disappearances of Australian Frogs, Canberra, Australia: Environment Australia, pp 109–130Google Scholar
  19. Green DE, Converse KA, Schrader AK (2002) Epizootiology of sixty-four amphibian morbidity and mortality events in the USA, 1996–2001. Annals of the New York Academy of Science 969:323–339CrossRefGoogle Scholar
  20. Hayes T, Collins A, Lee M, Mendoza M, Noriega N, Stuart AA, et al. (2002) Hermaphroditic, demasculinized frogs after exposure to the herbicide atrazine at low ecologically relevant doses. Proceedings of the National Academy of Science 99:5476–5480CrossRefGoogle Scholar
  21. Hero J-M (1989) A simple code for toe clipping anurans. Herpetological Review 20:66–67Google Scholar
  22. Hero J-M, Morrison C (2004) Frog declines in Australia: global implications. The Herpetological Journal 14:175–186Google Scholar
  23. Jensen JB, Camp CD (2003) Human exploitation of amphibians: direct and indirect impacts. In: Semlitsch RD (editor), Amphibian Conservation, Washington, DC: Smithsonian Institution, pp 199–213Google Scholar
  24. Kats LB, Ferrer RP (2003) Alien predators and amphibian declines: review of two decades of science and the transition to conservation. Diversity and Distributions 9:99–110CrossRefGoogle Scholar
  25. Kriger KM, Hero J-M (2006) Large-scale seasonal variation in the prevalence and severity of chytridiomycosis. Journal of Zoology (in press)Google Scholar
  26. Kriger KM, Hines H, Hyatt AD, Boyle DG, Hero J-M (2006a) Techniques for detecting chytridiomycosis in wild frogs: comparing histology with real-time Taqman PCR. Diseases of Aquatic Organisms (in press)Google Scholar
  27. Kriger KM, Hero J-M, Ashton KJ (2006b) Cost efficiency in the detection of chytridiomycosis using PCR assay. Diseases of Aquatic Organisms (in press)Google Scholar
  28. La Marca E, Lips KR, Lotters S (2005) Catastrophic population declines and extinctions in neotropical Harlequin frogs (Bufonidae: Atelopus). Biotropica 37:190–201CrossRefGoogle Scholar
  29. Lamirande EW, Nichols DK (2002) Effects of host age on susceptibility to cutaneous chytridiomycosis in blue-and-yellow poison dart frogs (Dendrobates tinctorius). Proceedings of the Sixth International Symposium on the Pathology of Reptiles and Amphibians, St. Paul, Minnesota, USAGoogle Scholar
  30. Lannoo MJ, Lang K, Waltz T, Phillips GS (1994) An altered amphibian assemblage: Dickinson county, Iowa, 70 years after Frank Blanchard’s survey. American Midland Naturalist 131:311–319CrossRefGoogle Scholar
  31. Lips KR (1999) Mass mortality and population declines of anurans at an upland site in western Panama. Conservation Biology 13:117–125CrossRefGoogle Scholar
  32. Lips KR, Brem F, Brenes R, Reeve JD, Alford RA, Voyles J, et al. (2006) Emerging infectious disease and the loss of biodiversity in a neotropical amphibian community. Proceedings of the National Academy of Science 103:3165–3170CrossRefGoogle Scholar
  33. Longcore JE, Pessier AP, Nichols DK (1999) Batrachochytrium dendrobatidis gen. et sp. nov., a chytrid pathogenic to amphibians. Mycologia 91:219–227CrossRefGoogle Scholar
  34. Marantelli G, Berger L, Speare R, Keegan L (2004) Changes in distribution of Batrachochytrium dendrobatidis and keratin during tadpole development leading to high mortality after metamorphosis. Pacific Conservation Biology 10:173–179Google Scholar
  35. May RM, Anderson RM (1983) Epidemiology and genetics in the coevolution of parasites and hosts. Proceedings of the Royal Society of London. Series B. Biological Sciences 219:281–313Google Scholar
  36. McDonald K, Alford RA (1999) A review of declining frogs in northern Queensland. In: Campbell A (editor), Declines and Disappearances of Australian Frogs, Canberra, Australia: Environment Australia, pp 14–22Google Scholar
  37. Nichols DK, Lamirande EW, Pessier AP, Longcore JE (2001) Experimental transmission of cutaneous chytridiomycosis in dendrobatid frogs. Journal of Wildlife Diseases 37:1–11Google Scholar
  38. Parris MJ, Cornelius TO (2004) Fungal pathogen causes competitive and developmental stress in larval amphibian communities. Ecology 85:3385–3395CrossRefGoogle Scholar
  39. Pounds JA (2001) Climate and amphibian declines. Nature 410:639–640CrossRefGoogle Scholar
  40. Pounds JA, Bustamante MR, Coloma LA, Consuegra JA, Fogden MPL, Foster PN, et al. (2006) Widespread amphibian extinctions from epidemic disease driven by global warming. Nature 439:161–167CrossRefGoogle Scholar
  41. Quimby FW, Casey AC, Arquette MF (2005) From dogs to frogs: how pets, laboratory animals, and wildlife aided in elucidating harmful effects arising from a hazardous dumpsite. ILAR 46:364–369Google Scholar
  42. Retallick R, McCallum H, Speare R (2004) Endemic infection of the amphibian chytrid fungus in a frog community post-decline. Public Library of Science 2:1–7Google Scholar
  43. Rollins-Smith LA, Conlon JM (2005) Antimicrobial peptide defenses against chytridiomycosis, an emerging infectious disease of amphibian populations. Developmental and Comparative Immunology 29:589–598CrossRefGoogle Scholar
  44. Speare R, Berger L (2005) Chytridiomycosis in amphibians in Australia. Available: [accessed March 28, 2006]
  45. Stuart SN, Chanson JS, Cox NA, Young BE, Rodrigues ASL, Fischman DL, et al. (2004) Status and trends of amphibian declines and extinctions worldwide. Science 306:1783–1786CrossRefGoogle Scholar
  46. Weldon C, du Preez LH (2004) Decline of the Kihansi spray toad, Nectophrynoides asperginis, from the Udzungwa mountains, Tanzania. Froglog 62:2–3Google Scholar
  47. Williams SE, Bolitho EE, Fox S (2003) Climate change in Australian tropical forests: an impending environmental catastrophe. Proceedings of the Royal Society of London Series B. Biological Sciences 270:1887–1892Google Scholar
  48. Woodhams DC, Rollins-Smith LA, Carey C, Reinert L, Tyler MJ, Alford RA (2005) Population trends associated with skin peptide defences against chytridiomycosis in Australian frogs. Oecologia 146:531–540CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  1. 1.Centre for Innovative Conservation StrategiesGriffith UniversityQueenslandAustralia

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