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Esophagus

, Volume 15, Issue 3, pp 198–204 | Cite as

The effect of platelet-rich plasma on motility changes in experimental caustic esophageal burn

  • Mustafa Onur Oztan
  • Sule Oztan
  • Neslihan Duzenli
  • Murat Olukman
  • Gokhan Koyluoglu
Original Article

Abstract

Background

Besides stricture formation, diminished esophageal motility after caustic esophageal burns also plays a role in the deterioration of the clinical course. In this study, we aimed to investigate the effect of caustic burn on the esophageal contractions and the effect of platelet-rich plasma (PRP) on these changes.

Methods

Twenty-one Wistar albino rats were divided into three groups [Sham operation (n = 8), caustic esophageal burn with NaOH (n = 6), PRP treatment after caustic burn (n = 7)]. After 3 weeks, esophagectomy was performed and contractions and EFS responses were evaluated in the organ bath.

Results

KCl- and acetylcholine-induced responses were reduced in the Burn group, but increased in Sham and PRP groups (p < 0.05). EFS responses were higher in Burn group compared to the other groups. Response with l-arginine was increased in Burn group, but decreased in PRP group. There was more decrease in the contraction in Sham and PRP groups compared to the Burn group after SNP (sodium nitroprusside) incubation (p < 0.05). L-NAME (Nω-Nitro-l-arginine methyl ester) did not change the EFS responses in the Burn group, but EFS responses were decreased significantly in Sham and PRP groups (p < 0.05). EFS responses were decreased in all groups, but more in the Sham and PRP groups after Y-27632 (Rho-kinase inhibitor) incubation (p < 0.05).

Conclusions

For the first time, we demonstrated that both cholinergic and non-adrenergic non-cholinergic mechanisms are responsible for the altered motility in corrosive esophageal injury. Our results suggest that PRP treatment may be helpful in regulating the esophageal motility and decreasing altered contractions in corrosive burns. This effect may also contribute to the reduction of stricture formation, especially by reducing inappropriate contractions of the esophageal wall during the post-burn healing phase.

Keywords

Caustic burn Esophageal motility Non-adrenergic non-cholinergic system Platelet-rich plasma 

Notes

Funding

The financial support for this study is provided from the Scientific Research Fund of Katip Celebi University (Project number: 2016-0027).

Compliance with ethical standards

Ethical statement

This study was approved by the Local Ethics Committee of our university and conformed to the Guide for the Care and Use of Laboratory Animals (US National Institutes of Health Publication no: 85-23, revised 1996). Our work conforms to the guidelines set forth in the Helsinki Declaration of 1975, as revised in 2000, concerning Human and Animal Rights, and that they followed the policy concerning Informed Consent.

Conflict of interest

The authors also declare that they have no conflicts of interest and any financial agreements with pharmaceutical or biomedical firms whose products are pertinent to the subject matter dealt within the manuscript.

References

  1. 1.
    Rothstein FC. Caustic injuries to esophagus in children. Pediatr Clin N Am. 1986;33:665–74.CrossRefGoogle Scholar
  2. 2.
    Kay M, Wyllie R. Caustic ingestions in children. Curr Opin Pediatr. 2009;21:651–4.CrossRefPubMedGoogle Scholar
  3. 3.
    Dantas RO, Mamede RC. Esophageal motility in patients with esophageal caustic injury. Am J Gastroenterol. 1996;91:1157–61.PubMedGoogle Scholar
  4. 4.
    Tugay M, Utkan T, Utkan Z. Effects of caustic lye injury to the esophageal smooth muscle reactivity: in vitro study. J Surg Res. 2003;113:128–32.CrossRefPubMedGoogle Scholar
  5. 5.
    Marx RE. Platelet-rich plasma (PRP): what is PRP and what is not PRP? Implant Dent. 2001;10:225–8.CrossRefPubMedGoogle Scholar
  6. 6.
    Anitua E, Andia I, Ardanza B, et al. Autologous platelets as a source of proteins for healing and tissue regeneration. Thromb Haemost. 2004;91:4–15.PubMedGoogle Scholar
  7. 7.
    Dhillon RS, Schwarz EM, Maloney MD. Platelet-rich plasma therapy—future or trend? Arthritis Res Ther. 2012;14:219.CrossRefPubMedPubMedCentralGoogle Scholar
  8. 8.
    Klosová H, Stětinský J, Bryjová I, et al. Objective evaluation of the effect of autologous platelet concentrate on post-operative scarring in deep burns. Burns. 2013;39:1263–76.CrossRefPubMedGoogle Scholar
  9. 9.
    Boswell SG, Cole BJ, Sundman EA, et al. Platelet-rich plasma: a milieu of bioactive factors. Arthroscopy. 2012;28:429–39.CrossRefPubMedGoogle Scholar
  10. 10.
    Lupa M, Magne J, Guarisco JL, et al. Update on the diagnosis and treatment of caustic ingestion. Ochsner J. 2009;9:54–9.PubMedPubMedCentralGoogle Scholar
  11. 11.
    Contini S, Scarpignato C. Caustic injury of the upper gastrointestinal tract: a comprehensive review. World J Gastroenterol. 2013;19:3918–30.CrossRefPubMedPubMedCentralGoogle Scholar
  12. 12.
    Marlow SL, Blennerhassett MG. Deficient innervation characterizes intestinal strictures in a rat model of colitis. Exp Mol Pathol. 2006;80:54–66.CrossRefPubMedGoogle Scholar
  13. 13.
    Okata Y, Hisamatsu C, Nishijima E, et al. Topical application of basic fibroblast growth factor reduces esophageal stricture and esophageal neural damage after sodium hydroxide-induced esophagitis in rats. Pediatr Surg Int. 2012;28:43–9.CrossRefPubMedGoogle Scholar
  14. 14.
    Kajikawa Y, Morihara T, Sakamoto H, et al. Platelet-rich plasma enhances the initial mobilization of circulation-derived cells for tendon healing. J Cell Physiol. 2008;215:837–45.CrossRefPubMedGoogle Scholar
  15. 15.
    Gehanno P, Guedon C, Marche C, et al. Prevention of caustic stenosis of the esophagus by d-penicillamine: research using an experimental model. Nouv Presse Med. 1978;7:3944.PubMedGoogle Scholar
  16. 16.
    Okata Y, Hisamatsu C, Hasegawa T, et al. Development of a model of benign esophageal stricture in rats: the optimal concentration of sodium hydroxide for stricture formation. Pediatr Surg Int. 2011;27:73–80.CrossRefPubMedGoogle Scholar
  17. 17.
    Harnett KM, Cao W, Kim N, et al. Signal transduction in esophageal and LES circular muscle contraction. Yale J Biol Med. 1999;72:153–68.PubMedPubMedCentralGoogle Scholar
  18. 18.
    Shin CY, La HO, Lee YP, et al. The alteration of intracellular signaling on the smooth muscle cells contraction in cat esophagitis. Life Sci. 2004;74:2199–211.CrossRefPubMedGoogle Scholar
  19. 19.
    Bult H, Boeckxstaens GE, Pelckmans PA, et al. Nitric oxide as an inhibitory non-adrenergic non-cholinergic neurotransmitter. Nature. 1990;345:346–7.CrossRefPubMedGoogle Scholar
  20. 20.
    Moncada S, Higgs A. The l-Arginine-nitric oxide pathway. N Engl J Med. 1993;329:2002–12.CrossRefPubMedGoogle Scholar
  21. 21.
    Wu G, Morris SM. Arginine metabolism: nitric oxide and beyond. Biochem J. 1998;336:1–17.CrossRefPubMedPubMedCentralGoogle Scholar
  22. 22.
    Chakder S, Rattan S. l-Arginine deficiency causes suppression of nonadrenergic noncholinergic nerve-mediated smooth muscle relaxation: role of L-citrulline recycling. J Pharmacol Exp Ther. 1997;282:378–84.PubMedGoogle Scholar
  23. 23.
    Kohjitani A, Miyawaki T, Funahashi M, et al. Intravenous anesthetics inhibit nonadrenergic noncholinergic lower esophageal sphincter relaxation via nitric oxide-cyclic guanosine monophosphate pathway modulation in rabbits. Anesthesiology. 2001;95:176–83.CrossRefPubMedGoogle Scholar
  24. 24.
    Martínez-Cuesta MA, Esplugues JV, Whittle BJR. Modulation by nitric oxide of spontaneous motility of the rat isolated duodenum: role of tachykinins. Br J Pharmacol. 1996;118:1335–40.CrossRefPubMedPubMedCentralGoogle Scholar
  25. 25.
    Grossi L, Falcucci M, Lapenna D, et al. Effect of nitric oxide on propagated clusters of spontaneous motor waves in an ex vivo rabbit intestinal preparation. Neurogastroenterol Motil. 1996;8:201–5.CrossRefPubMedGoogle Scholar
  26. 26.
    Ragy M, Elbassuoni E. The role of nitric oxide and L-type calcium channel blocker in the contractility of rabbit ileum in vitro. J Physiol Biochem. 2012;68:521–8.CrossRefPubMedGoogle Scholar
  27. 27.
    Farré R, Sifrim D. Regulation of basal tone, relaxation and contraction of the lower oesophageal sphincter. Relevance to drug discovery for oesophageal disorders. Br J Pharmacol. 2008;153:858–69.CrossRefPubMedGoogle Scholar
  28. 28.
    Ceylan H, Yapici S, Tutar E, et al. Protective effects of dexpanthenol and y-27632 on stricture formation in a rat model of caustic esophageal injury. J Surg Res. 2011;171:517–23.CrossRefPubMedGoogle Scholar
  29. 29.
    Ishizaki T, Uehata M, Tamechika I, et al. Pharmacological properties of Y-27632, a specific inhibitor of rho-associated kinases. Mol Pharmacol. 2000;57:976–83.PubMedGoogle Scholar
  30. 30.
    Sundman EA, Cole BJ, Karas V, et al. The anti-inflammatory and matrix restorative mechanisms of platelet-rich plasma in osteoarthritis. Am J Sports Med. 2014;42:35–41.CrossRefPubMedGoogle Scholar
  31. 31.
    Carter MJ, Fylling CP, Parnell LK. Use of platelet rich plasma gel on wound healing: a systematic review and meta-analysis. Eplasty. 2011;11:e38.PubMedPubMedCentralGoogle Scholar

Copyright information

© The Japan Esophageal Society and Springer Japan KK, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Pediatric Surgery, Katip Celebi Universitesi Dekanligi, Cocuk Cerrahisi Anabilim DaliIzmir Katip Çelebi UniversityCigliTurkey
  2. 2.MEST Aesthetic and Plastic Surgery CenterKonakTurkey
  3. 3.Department of Medical Pharmacology, Ege Universitesi DekanligiEge UniversityBornovaTurkey

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