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Comparison of retinal vessel measurements using adaptive optics scanning laser ophthalmoscopy and optical coherence tomography

  • Clinical Investigation
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Abstract

Purpose

We compared adaptive optics scanning laser ophthalmoscopy (AOSLO) and optical coherence tomography (OCT) vessel caliber measurements.

Methods

AOSLO videos were acquired from 28 volunteers with healthy eyes. Artery measurements were made 0.5–1 disc diameters away from the optic disc margin. Individual segmented retinal arterial caliber was measured in synchronization with cardiac pulsation and averaged to obtain final horizontal retinal arterial caliber (ACH) and horizontal retinal arterial lumen (ALH). All OCT images were obtained with the Spectralis OCT, a spectral-domain OCT system. Vertical retinal arterial caliber (ACV) and vertical retinal arterial lumen (ALV) were measured on the same artery measured with AOSLO. Measurements made with the two imaging systems were compared.

Results

Average ACH, measured with AOSLO, was 123.4 ± 11.2 and average ALH was 101.8 ± 10.2 µm. Average ACV, measured with OCT, was 125.5 ± 11.4 and average ALV was 99.1 ± 10.6 µm. Both arterial caliber (r = 0.767, p < 0.0001) and arterial lumen (r = 0.81, p < 0.0001) measurements were significantly correlated between imaging modalities. Additionally, ACH and ACV were not significantly different (p = 0.16). However, ALH measurements were significantly higher than ALV measurements (p = 0.03).

Conclusions

Vessel measurements made with AOSLO and OCT were well correlated. Moreover, plasma is visible and distinguishable from the retinal vessel wall in AOSLO images but not in OCT images. Therefore, AOSLO may measure vessel width more precisely than OCT.

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Acknowledgments

This work was supported, in part, by the Innovative Techno-Hub for Integrated Medical Bio-Imaging of the Project for Developing Innovation Systems, from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) in Japan.

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Authors and Affiliations

  1. Department of Ophthalmology and Visual Sciences, Kyoto University Graduate School of Medicine, 54 Shogoin Kawahara-cho, Sakyo-ku, Kyoto, 606-8507, Japan

    Shigeta Arichika, Akihito Uji, Sotaro Ooto, Yuki Muraoka & Nagahisa Yoshimura

Authors
  1. Shigeta Arichika
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  2. Akihito Uji
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  3. Sotaro Ooto
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  4. Yuki Muraoka
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  5. Nagahisa Yoshimura
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Corresponding author

Correspondence to Akihito Uji.

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Conflicts of interest

S. Arichika, None; A. Uji, None; S. Ooto, None; Y. Muraoka, None; N. Yoshimura, Financial support (Topcon Corporation, Nidek, Canon), Consultant (Nidek).

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Arichika, S., Uji, A., Ooto, S. et al. Comparison of retinal vessel measurements using adaptive optics scanning laser ophthalmoscopy and optical coherence tomography. Jpn J Ophthalmol 60, 166–171 (2016). https://doi.org/10.1007/s10384-016-0435-3

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  • DOI: https://doi.org/10.1007/s10384-016-0435-3

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