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Japanese Journal of Ophthalmology

, Volume 57, Issue 1, pp 80–84 | Cite as

Mapping owl’s eye cells of patients with cytomegalovirus corneal endotheliitis using in vivo laser confocal microscopy

  • Hideaki Yokogawa
  • Akira KobayashiEmail author
  • Kazuhisa Sugiyama
Clinical Investigation

Abstract

Purpose

To produce a two-dimensional reconstruction map of owl’s eye cells using in vivo laser confocal microscopy in patients with cytomegalovirus (CMV) corneal endotheliitis, and to demonstrate any association between owl’s eye cells and coin-shaped lesions observed with slit-lamp biomicroscopy.

Method

Two patients (75- and 77-year-old men) with polymerase chain reaction-proven CMV corneal endotheliitis were evaluated in this study. Slit-lamp biomicroscopy and in vivo laser confocal microscopy were performed. Images of owl’s eye cells in the endothelial cell layer were arranged and mapped into subconfluent montages. Montage images of owl’s eye cells were then superimposed on a slit-lamp photo of the corresponding coin-shaped lesion. Degree of concordance between the confocal microscopic images and slit-lamp photos was evaluated.

Results

In both eyes, a two-dimensional reconstruction map of the owl’s eye cells was created by computer software using acquired confocal images; the maps showed circular patterns. Superimposing montage images of owl’s eye cells onto the photos of a coin-shaped lesion showed good concordance in the two eyes.

Conclusions

This study suggests that there is an association between owl’s eye cells observed by confocal microscopy and coin-shaped lesions observed by slit-lamp biomicroscopy in patients with CMV corneal endotheliitis. The use of in vivo laser confocal microscopy may provide clues as to the underlying causes of CMV corneal endotheliitis.

Keywords

Cytomegalovirus corneal endotheliitis Confocal microscopy Owl’s eye cell Coin-shaped lesion 

Notes

Acknowledgments

This study was supported by a grant from the Intractable Disease Treatment Research Program (10103447), the Ministry of Health, Labour and Welfare, Japan, and a Grant-in-Aid for Scientific Research (KAKENHI), Japan (Nos. 22591934, 23890072).

Conflict of interest

No authors have any financial/conflicting interests to disclose.

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Copyright information

© Japanese Ophthalmological Society 2012

Authors and Affiliations

  • Hideaki Yokogawa
    • 1
  • Akira Kobayashi
    • 1
    Email author
  • Kazuhisa Sugiyama
    • 1
  1. 1.Department of OphthalmologyKanazawa University Graduate School of Medical ScienceKanazawaJapan

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