Abstract
Purpose
To investigate the protective effects of d-allose, a rare sugar, on pressure-induced ischemia during vitrectomy in the rabbit eye.
Methods
The rabbits underwent pars plana vitrectomy, and continuous intraocular irrigation at a perfusion pressure of 140 mmHg was performed for 45 min. Intraocular pressure was regulated by adjusting the height of a bottle of balanced saline solution containing d-allose. Morphometric studies were performed to study the effects of d-allose on the histological changes induced by ischemia in the rabbit retina. Electroretinograms (ERGs) were taken before and 1 and 7 days after vitrectomy. Nitroblue tetrazolium was used as an index of superoxide anion (O ·−2 ) generation. Data were analyzed by use of the unpaired Student’s t test.
Results
Seven days after ischemia, significant reductions in both number of ganglion cells and the thickness of the inner plexiform layer were observed. d-Allose significantly inhibited ischemic injury of the inner retina (P < 0.05). On postoperative day 7, amplitudes of ERG b-waves were significantly lower in the control group than in the d-allose group (P < 0.05). d-Allose suppressed the production of O2 −.
Conclusions
Intraocular irrigation with d-allose during vitrectomy may protect the retina against ischemia-induced damage.
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Acknowledgments
This work was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology of Japan (20592078).
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Mizote, M., Hirooka, K., Fukuda, K. et al. d-Allose as ischemic retina injury inhibitor during rabbit vitrectomy. Jpn J Ophthalmol 55, 294–300 (2011). https://doi.org/10.1007/s10384-011-0012-8
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DOI: https://doi.org/10.1007/s10384-011-0012-8