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Pharmacological dissection of multifocal electroretinograms of rabbits with Pro347Leu rhodopsin mutation

  • Laboratory Investigation
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Abstract

Purpose

To determine whether photoreceptor degeneration in transgenic (Tg) rabbits carrying the Pro347Leu rhodopsin mutation alters the neural activity of the middle and inner retinal neurons.

Methods

Multifocal electroretinograms (mfERGs) were recorded from eight 12-week-old Tg rabbits both before and after intravitreal injection of the following: tetrodotoxin citrate (TTX), N-methyl-dl-aspartic acid (NMDA), 2-amino-4-phosphonobutyric acid (APB), and cis-2,3-piperidine-dicarboxylic acid (PDA). Digital subtraction of the mfERGs recorded after the drugs were administered from those recorded before was used to extract the components that were eliminated by these drugs. Eight agematched, wild-type (WT) rabbits were studied with the same protocol.

Results

There was no reduction in the amplitude of the cone photoreceptor response of the mfERGs in Tg rabbits. Both the first positive and the first negative waves of the ON-bipolar cell responses were significantly larger in the Tg than in the WT rabbits. Late negative waves of the ON-bipolar cell response were recorded only in the WT rabbits. The first negative wave of the inner retinal responses was larger in the Tg than in the Wt rabbits. The late positive waves were seen mainly in the WT rabbits.

Conclusions

The ON-bipolar cell and inner retinal responses were altered at the early stage of photoreceptor degeneration in Tg rabbits despite the preservation of the cone photoreceptor responses.

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Authors and Affiliations

  1. Department of Ophthalmology, Iwate Medical University School of Medicine, Morioka, Iwate, Japan

    Daisuke Yokoyama, Shigeki Machida, Tomoharu Nishimura & Daijiro Kurosaka

  2. Department of Ophthalmology, Nagoya University Graduate School of Medicine, Nagoya, Japan

    Mineo Kondo & Hiroko Terasaki

  3. Department of Ophthalmology, Iwate Medical University School of Medicine, 19-1 Uchimaru, Morioka, Iwate, 020-8505, Japan

    Shigeki Machida

Authors
  1. Daisuke Yokoyama
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  2. Shigeki Machida
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  3. Mineo Kondo
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  4. Hiroko Terasaki
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  5. Tomoharu Nishimura
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  6. Daijiro Kurosaka
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Corresponding author

Correspondence to Shigeki Machida.

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Yokoyama, D., Machida, S., Kondo, M. et al. Pharmacological dissection of multifocal electroretinograms of rabbits with Pro347Leu rhodopsin mutation. Jpn J Ophthalmol 54, 458–466 (2010). https://doi.org/10.1007/s10384-010-0842-9

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  • DOI: https://doi.org/10.1007/s10384-010-0842-9

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