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Comparison of the influence of intracameral gentamicin, gatifloxacin, and moxifloxacin on the corneal endothelium in a rabbit model

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Abstract

Purpose

To compare the effect of three intracameral antibiotics, gentamicin (GM), gatifloxacin (GFLX), and moxifloxacin (MFLX), on the rabbit corneal endothelium.

Methods

Twenty-four eyes from 18 rabbits were used. In the GM treatment group of 12 eyes, a dose of 20 mg/ml, 2 mg/ml, 200 μg/ml, or 20 μg/ml of GM was injected into the anterior chamber. In the GFLX and MFLX treatment groups were injected into the anterior chamber of three eyes. The central corneal thickness was measured. The eyes were then enucleated for observation under scanning electron microscopy.

Results

Three days after the intracameral injection, a significant difference in central corneal thickness was found between the GM 20 mg/ml group and the control group (P < 0.05), but not between any other groups. The damage rate at the endothelial cell level was 67% in the GM 20 mg/ml group, 56% in the GM 2 mg/ml group, 33% in the GM 200 μg/ml group, 22% in the GM 20 μg/ml group, 22% in the GFLX group, and 0% in the MFLX group.

Conclusions

Intracameral GFLX or MFLX was almost nontoxic to the rabbit corneal endothelium, in contrast to the toxic results of intracameral GM 20 and 2 mg/ml.

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Authors and Affiliations

  1. Department of Ophthalmology, School of Medicine, Toho University, Tokyo, Japan

    Shinichiro Kobayakawa & Tetsuo Tochikubo

  2. Department of Ophthalmology, School of Medicine, Juntendo University, Tokyo, Japan

    Yoshimune Hiratsuka, Yasuo Watabe & Akira Murakami

  3. Department of Ophthalmology, School of Medicine, Toho University, 7-5-23 Omori-nishi, Ohta-ku, Tokyo, 143-8541, Japan

    Shinichiro Kobayakawa

Authors
  1. Shinichiro Kobayakawa
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  2. Yoshimune Hiratsuka
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  3. Yasuo Watabe
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  4. Akira Murakami
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  5. Tetsuo Tochikubo
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Correspondence to Shinichiro Kobayakawa.

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Kobayakawa, S., Hiratsuka, Y., Watabe, Y. et al. Comparison of the influence of intracameral gentamicin, gatifloxacin, and moxifloxacin on the corneal endothelium in a rabbit model. Jpn J Ophthalmol 54, 481–485 (2010). https://doi.org/10.1007/s10384-010-0838-5

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  • DOI: https://doi.org/10.1007/s10384-010-0838-5

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