Abstract
Purpose
This was a quantitative study to investigate the minimum endotoxin concentration causing inflammation in the anterior segment of the eye.
Methods
Endotoxin was injected intracamerally in pigmented rabbits. A quantitative determination of flare and cells in the aqueous was performed using a laser flare-cell photometer, before and until 72 h after the treatment. An area under the curve (AUC) analysis was employed to evaluate the whole inflammatory reaction regarding flare values.
Results
The time course of flare values in each endotoxin group showed a similar pattern with a peak value at 3 h. An AUC corresponding to values for “average +2σ”, 19301.8 in control eyes, was considered the cutoff value. Using this cutoff value and the regression curve in endotoxin-treated groups, the minimum endotoxin concentration causing inflammation regarding flare values was determined to be 0.60 endotoxin units (EU). Cell counts (cells/0.5 mm3·0.5 s) corresponding to the value “average +2σ”, 6.07 at 24 h, in control eyes was considered to be the cutoff value. The minimum endotoxin concentration regarding cell counts was determined to be 0.23 EU.
Conclusion
There was a dissociation in response between flare and cells in the aqueous to intracameral endotoxin. The minimum endotoxin concentration causing inflammation ranged between 0.23 and 0.60 EU.
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References
Neumann AC, McCarty GR, Sanders DR, Raanan MG. Small incisions to control astigmatism during cataract surgery. J Cataract Refract Surg 1989;15:78–84.
Pearce JL. Intraocular lenses. Curr Opin Ophthalmol 1992;3:29–38.
Lundgren B, Holst A, Tärnholm A, Rolfsen W. Cellular reaction following cataract surgery with implantation of the heparin-surface-modified intraocular lens in rabbits with experimental uveitis. J Cataract Refract Surg 1992;18:602–606.
Sawa M, Masuda K. Topical indomethacin in soft cataract aspiration. Jpn J Ophthalmol 1976;20:514–519.
Mochizuki M, Sawa M, Masuda K. Topical indomethacin in intracapsular extraction of senile cataract. Jpn J Ophthalmol 1977;21:215–226.
Hellinger WC, Hasan SA, Bacalis LP, et al. Outbreak of toxic anterior segment syndrome following cataract surgery associated with impurities in autoclave steam moisture. Infect Control Hosp Epidemiol 2006;27:294–298.
Mathys KC, Cohen KL, Bagnell CR. Identification of unknown intraocular material after cataract surgery: evaluation of a potential cause of toxic anterior segment syndrome. J Cataract Refract Surg 2008;34:465–469.
Kim SY, Park YH, Kim HS, Lee YC. Bilateral toxic anterior segment syndrome after cataract surgery. Can J Ophthalmol 2007;42:490–491.
Holland SP, Morck DW, Lee TL. Update on toxic anterior segment syndrome. Curr Opin Ophthalmol 2007;18:4–8.
Rosenbaum JT, McDevitt HO, Guss RB, et al. Endotoxin-induced uveitis in rats as a model for human disease. Nature 1980;286:611–613.
Jacobs DR, Cohen HB. The inflammatory role of endotoxin in rabbit gram-negative bacterial endophthalmitis. Invest Ophthalmol Vis Sci 1984;25:1074–1079.
Green K, Paterson CA, Cheeks L, et al. Ocular blood flow and vascular permeability in endotoxin-induced inflammation. Ophthalmic Res 1990;22:287–294.
McGahan MC, Fleisher LN. Cellular response to intravitreal injection of endotoxin and xanthine oxidase in rabbits. Graefes Arch Clin Exp Ophthalmol 1992;230:463–467.
Metrikin DC, Wilson CA, Berkowitz BA, et al. Measurement of blood-retinal barrier breakdown in endotoxin-induced endophthalmitis. Invest Ophthalmol Vis Sci 1995;36:1361–1370.
Howes EL Jr, Aronson SB, McKay DG. Ocular vascular permeability. Effect of systemic administration of bacterial endotoxin. Arch Ophthalmol 1970;84:360–367.
Williams RN, Paterson CA. PMN accumulation in aqueous humor and iris-ciliary body during intraocular inflammation. Invest Ophthalmol Vis Sci 1984;25:105–108.
Csukas S, Paterson CA, Brown K, et al. Time course of rabbit ocular inflammatory response and mediator release after intravitreal endotoxin. Invest Ophthalmol Vis Sci 1990;31:382–387.
Sawa M, Tsurimaki Y, Tsuru T, Shimizu H. New quantitative method to determine protein concentration and cell number in aqueous in vivo. Jpn J Ophthalmol 1988;32:132–142.
Obata T, Nomura M, Kase Y, Sasaki H, Shirasawa Y. Early detection of the Limulus amebocyte lysate reaction evoked by endotoxins. Anal Biochem 2008;373:281–286.
Hogan MJ, Kimura SJ, Thygeson P. Signs and symptoms of uveitis. I. Anterior uveitis. Am J Ophthalmol 1959;47:155–170.
Oshika T, Nishi M, Mochizuki M, et al. Quantitative assessment of aqueous flare and cells in uveitis. Jpn J Ophthalmol 1989;33:279–287.
Goto H, Mochizuki M, Yamaki K, et al. Epidemiological survey of intraocular inflammation in Japan. Jpn J Ophthalmol 2007;51:41–44.
Inoue Y, Usui M, Ohashi Y, Shiota H, Yamazaki T. Preoperative disinfection of the conjunctival sac with antibiotics and iodine compounds: a prospective randomized multicenter study. Jpn J Ophthalmol 2008;52:151–161.
Stjernschantz J. Autocoids and neuropeptides. In: Sears ML, editor. Pharmacology of the eye. Berlin: Springer; 1984, p. 311–366.
Miyake K. Prophylaxis of aphakic cystoid macular edema using topical indomethacin. J Am Intraocul Implant Soc 1978;4:174–179.
Takeo S, Watanabe Y, Suzuki M, Kadonosono K. Wavefront analysis of acrylic spherical and aspherical intraocular lenses. Jpn J Ophthalmol 2008;52:250–254.
Sawa M: Clinical application of laser flare-cell meter. Jpn J Ophthalmol 1990;34:346–363.
Ambache N. The use and limitations of atropine for pharmacological studies on autonomic effectors. Pharmacol Rev 1955;7:467–494.
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Sakimoto, A., Sawa, M., Oshida, T. et al. Minimum endotoxin concentration causing inflammation in the anterior segment of rabbit eyes. Jpn J Ophthalmol 53, 425–432 (2009). https://doi.org/10.1007/s10384-009-0683-6
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DOI: https://doi.org/10.1007/s10384-009-0683-6