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Induction of Connective Tissue Growth Factor in Retinal Pigment Epithelium Cells by Oxidative Stress

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Abstract

Purpose

To investigate whether connective tissue growth factor (CTGF) is upregulated in the retinal pigment epithelium (RPE) by oxidative stress in vitro and in vivo.

Methods

ARPE19 cells were subjected to two forms of oxidative stress: hypoxia/reoxygenation and paraquat exposure. CTGF expression was studied by real-time reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blot analysis. The ocular expression of CTGF was analyzed by RT-PCR and immunohistochemistry in an in vivo murine model of oxidative stress exposed to light after intravenous injection of rose bengal.

Results

CTGF mRNA expression in ARPE19 cells was upregulated by oxidative stress and peaked 1 h after reoxygenation; protein expression peaked 2 h after reoxygenation. Using paraquat, both CTGF mRNA and protein were upregulated by five doses of paraquat. In vivo, CTGF was upregulated when evaluated by RT-PCR and immunohistochemistry using murine retinas stimulated by oxidative stress.

Conclusions

Upregulation of CTGF expression was confirmed in RPE cells by oxidative stress in vitro and in vivo. CTGF might be one of the factors that induces the changes observed in aging retinas. Jpn J Ophthalmol 2006;50:229–234 © Japanese Ophthalmological Society 2006

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Authors and Affiliations

  1. Department of Anatomy and Neuroscience, Osaka University Graduate School of Medicine, Osaka, Japan

    Satoshi Matsuda, Taiichi Katayama, Yoshihisa Koyama & Masaya Tohyama

  2. Department of Ophthalmology, Osaka University Graduate School of Medicine, Osaka, Japan

    Satoshi Matsuda, Fumi Gomi & Yasuo Tano

Authors
  1. Satoshi Matsuda
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  2. Fumi Gomi
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  3. Taiichi Katayama
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  4. Yoshihisa Koyama
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  5. Masaya Tohyama
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  6. Yasuo Tano
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Correspondence to Fumi Gomi.

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Cite this article

Matsuda, S., Gomi, F., Katayama, T. et al. Induction of Connective Tissue Growth Factor in Retinal Pigment Epithelium Cells by Oxidative Stress. Jpn J Ophthalmol 50, 229–234 (2006). https://doi.org/10.1007/s10384-005-0317-6

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  • DOI: https://doi.org/10.1007/s10384-005-0317-6

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