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Disodium Cromoglycate Inhibits Capsaicin-Induced Eosinophil Infiltration of Conjunctiva Independent of Mast Cells

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Abstract

Purpose

To investigate whether disodium cromoglycate (DSCG) inhibits capsaicin-induced eosinophil infiltration of the conjunctiva independent of mast cells.

Methods

We administered 5 µl of capsaicin solution (10−5 M) into the conjunctival sacs of mast cell-deficient W/Wv mice (12 animals) and wild-type mice (12 animals). As controls, the eyes of 12 wild-type and 12 W/Wv mice were treated with phosphate-buffered saline. Following treatment, the eyelids and eyeballs were removed en bloc at 3, 9, or 24 h, and were histologically examined. The number of infiltrated eosinophils and the expression of vascular cell adhesion molecule-l (VCAM-1) in the conjunctiva were quantified by the staining method of Hansel and immunohistochemical analysis. We also investigated whether treatment by depletion of neuropeptides or by DSCG administration could suppress the capsaicin-induced eosinophil infiltration of the conjunctiva.

Results

In both W/Wv and wild-type mice, eosinophil infiltration of conjunctival tissues was observed 3 h after capsaicin administration. In both strains of mice, the number of infiltrated eosinophils increased over time, with VCAM-1 expression on vascular endothelial cells peaking at 9 h after treatment, and decreasing gradually within 24 h after treatment. In both the neuropeptide-depleted and the DSCG-treated groups, eosinophil infiltration and VCAM-1 expression were suppressed in comparison with the nontreated group.

Conclusion

DSCG can directly inhibit neuropeptide-induced eosinophil infiltration of the conjunctiva independent of mast cells. Jpn J Ophthalmol 2006;50:205–210 © Japanese Ophthalmological Society 2006

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Authors and Affiliations

  1. Department of Ophthalmology, Juntendo University School of Medicine, Tokyo, Japan

    Nobuyuki Ebihara & Akira Murakami

  2. Post-marketing Development Research Center, Fujisawa Pharmaceutical Company, Ltd., Osaka, Japan

    Motoaki Nishikawa

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  1. Nobuyuki Ebihara
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  2. Motoaki Nishikawa
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  3. Akira Murakami
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Correspondence to Nobuyuki Ebihara.

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Ebihara, N., Nishikawa, M. & Murakami, A. Disodium Cromoglycate Inhibits Capsaicin-Induced Eosinophil Infiltration of Conjunctiva Independent of Mast Cells. Jpn J Ophthalmol 50, 205–210 (2006). https://doi.org/10.1007/s10384-005-0314-9

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  • DOI: https://doi.org/10.1007/s10384-005-0314-9

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