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Effects of everolimus on hepatic ischemia/reperfusion injury in an experimental rat model

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An Erratum to this article was published on 11 June 2013

Summary

Background

The current study is the first to evaluate the protective effect of everolimus on biochemical and histopathological features with an experimental hepatic ischemia reperfusion (I/R) injury model.

Methods

Twenty-four Wistar Albino rats were randomized into four groups: Group 1 (sham group) was subjected to laparotomy. Group 2 (I/R group) was subjected to ischemia for 1 hour and reperfusion for 24 hours without treatment. Group 3 was treated with 1.5 mg/kg/day everolimus perorally for 7 days without performing I/R. Group 4 was treated with 1.5 mg/kg/day everolimus perorally for 7 days followed with performing I/R. Blood samples and liver tissues were obtained to assess serum aspartate aminotransferase (AST), alanin aminotransferase (ALT), albumin, tumor necrosis factor-alpha (TNFα), interleukin-6 (IL-6) and tissue malonyl dialdehyde (MDA), and superoxide dismutase (SOD) levels and histopathologic examination.

Results

Serum AST and ALT levels of I/R group were significantly higher than sham group. Serum transaminase levels were significantly decreased after treatment with everolimus (group 4) when compared with I/R group (group 2). TNFα and MDA levels were decreased; however, SOD levels were increased in group 4. The sham group and group 3 showed normal histological findings. Morphologic analysis showed reduced degrees of injury in group 4 than group 2 with regards to cholestasis, sinusoidal dilatation, congestion, hydropic degeneration, and subcapsular necrosis.

Conclusions

This is the first report as regards everolimus attenuates biochemical and histopathological alterations of hepatic I/R injury. Everolimus can be a choice for preventing I/R injury during liver transplantation surgery in the future besides its well-known immunosuppressive property.

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Conflict of interest

The authors declare that the present study was funded by IMSED (Education Society of Internal Medicine After Graduation).

Bahar Gurlek Demirci, Mehmet Cindoruk, and Aydin Dalgic designed the research/study; Bahar Gurlek Demirci, Utku Tonguc Yilmaz, Ipek Isik Gonul, Ozlem Gulbahar, and Umut Demirci performed the research/study; Yilmaz performed the surgical procedures; Demirci and Yilmaz contributed important reagents; Demirci collected the data; Mehmet Derya Demirag analyzed the data; and Demirci wrote the paper.

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Authors and Affiliations

  1. Department of Nephrology, Baskent University Medical Faculty, Cankaya, 06520, Ankara, Turkey

    B. G. Demirci M.D.

  2. Department of Gastroenterology, Gazi University Medical Faculty, Ankara, Turkey

    M. Cindoruk

  3. Department of General Surgery, Gazi University Medical Faculty, Ankara, Turkey

    U. T. Yilmaz &  A. Dalgic

  4. Department of Rheumatology, Samsung Education and Research Hospital, Seoul, South Korea

    M. D. Demirag

  5. Department of Pathology, Gazi University Medical Faculty, Ankara, Turkey

    I. I. Gonul

  6. Department of Medical Oncology, Ataturk Education and Research Hospital, Ankara, Turkey

    U. Demirci

  7. Department of Biochemistry, Gazi University Medical Faculty, Ankara, Turkey

    O. Gulbahar

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  1. B. G. Demirci M.D.
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  2. M. Cindoruk
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  3. U. T. Yilmaz
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  4. M. D. Demirag
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  5. I. I. Gonul
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  6. U. Demirci
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  7. O. Gulbahar
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Correspondence to B. G. Demirci M.D..

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Demirci, B., Cindoruk, M., Yilmaz, U. et al. Effects of everolimus on hepatic ischemia/reperfusion injury in an experimental rat model. Eur Surg 44, 325–330 (2012). https://doi.org/10.1007/s10353-012-0152-1

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  • DOI: https://doi.org/10.1007/s10353-012-0152-1

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