Skip to main content

Advertisement

SpringerLink
Log in

Preservation injury of the small bowel graft in rats

Schädigung von Dünndarmtransplantaten der Ratte durch Perfusionslösungen

  • Original Scientific Paper
  • Published:
European Surgery Aims and scope Submit manuscript

Zusammenfassung

GRUNDLAGEN: Durch Perfusionslösungen kann es zu Transplantatschädigung kommen. Es trägt zur Entwicklung von postoperativen Komplikationen wie Organdysfunktion und Peritonitis bei und erhöht das Risiko der Abstoßung. Es werden geeignete Parameter gesucht, die den Transplantatsverletzungsgrad durch Perfusion und Ischämie oder Reperfusion objektivieren lassen. Morphologische Beurteilung von Veränderungen im Dünndarmtransplantat wird als "goldener Standard" verstanden, dessen Ergebnis als Basis für Bewertungen anderer Parameter dient. METHODIK: In dieser Studie wurden IL-2-Spiegel in der Perfusionslösung und im Effluat mit dem ELISA-quantitativen Set festgestellt. ERGEBNISSE: Mit der von uns gewählten Methode zeigt sich IL-2 als kein geeigneter Parameter für die Beurteilung des Verletzungsgrades durch Perfusionslösungen bei Ratten. SCHLUSSFOLGERUNGEN: Die untersuchten Parameter waren nicht geeignet, die UW- und HTK-Lösungen bezüglich einer möglichen Tranplantatschädigung zu vergleichen. Entsprechend unseren früheren Studien stellen die Morphologie und der Serotoninspiegel weiterhin besten Parameter für Bewertung des Transplantatverletzungsgrades durch Perfusionslösungen dar.

Summary

BACKGROUND: Preservation injury is a phenomenon adversely affecting the quality of transplant grafts. When attempting to identify its degree, suitable parameters are required. In an effort to find a feasible and clinically applicable parameter, we aim to evaluate the usability of IL-2 as a parameter to evaluate quantitatively preservation graft injury. METHODS: IL-2 levels in preservation solutions effluent including Histidin-Tryptophan-Ketoglutarate (HTK) and University of Wisconsin (UW) solutions used in rat (Wistar strain) small bowel graft preservation were determined using ELISA and Quantikine M rat IL-2 R2000. We investigated the effect of UW and HTK solutions on preservation injury of rat jejunal grafts during 0-, 1-, 6-, 9-, and 12-hour cold ischemia time (CIT). RESULTS: In spite of the presence of upwards trend line proportionate with the increase in CIT, these cumulative changes in IL-2 levels are not statistically significant and might not be specific. Although HTK effluent contained higher levels of IL-2 at 0, 6, and 12 hours CIT than UW effluent, these differences are statistically insignificant (p>0.05) CONCLUSIONS: While the outcome of this study is negative, it shows that IL-2 is not a suitable marker to determine the degree of small bowel graft preservation injury in rats.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Muller AR, Langrehr JM, Nalesnik M, Hoffman RA, Lee TK, Lee KKW, Schraut WH (1994) Mucosal glutaminase activity and histology as parameters of small bowel preservation injury. J Surg Res 56: 207

    Article  PubMed  CAS  Google Scholar 

  • Bragg LE, Thompson JS, West WW (1991) Intestinal diamine oxidase levels reflect ischemic injury. J Surg Res 50: 228

    Article  PubMed  CAS  Google Scholar 

  • Gollin G, Marks C, Marks WH (1993) Intestinal fatty acid binding protein in serum and urine reflects early ischemic injury to the small bowel. Surgery 113: 545

    PubMed  CAS  Google Scholar 

  • Hirata Y, Taguchi T, Suita S, Takeshige K (1994) Adenine nucleotide metabolism in relation to graft viability in rat small-bowel transplantation. Eur Surg Res 26: 309

    PubMed  CAS  Google Scholar 

  • Muller AR, Rao PN, Snyder JT, Hoffman RA, Schraut WH (1993) Hyaluronic acid and purine nucleoside phosphorylase in vascular and luminal effluents of small bowel grafts as parameters of preservation injury. Transplantation 55: 1225

    Google Scholar 

  • Taguchi T, Yamada T, Suita S, Ohta M (1995) The significance of cytological examination on reperfusion in rat small intestinal transplantation. Virchows Arch 427: 71

    Article  PubMed  CAS  Google Scholar 

  • Sasaki K, Park OJ, Bain A, Reilly KJ, Adamson WT, Koide S, Zhang W, Rombeau JL (1997) Glutamine protects function and improves preservation of small bowel segments. J Surg Res 73: 90

    Article  PubMed  CAS  Google Scholar 

  • Fabian MA, Bollinger RR, Wyble CW, Watkins WD, Canada AT (1991) Evaluation of solutions for small intestinal preservation. Biochemical changes as a function of storage time. Transplantation 52: 794

    Article  PubMed  CAS  Google Scholar 

  • Hamamoto I, Merhav H, Zhu Y, Suzuki M, Fujita S, Murase N, Todo S, Starzl TE (1992) Lipid peroxidation, brush border, and neutrophil enzyme activity after small bowel preservation: a comparison of preservation solutions. Transplant Proc 24: 1095

    PubMed  CAS  Google Scholar 

  • Schweizer E, Gassel A, Deltz E, Schroeder P (1992) Morphologic and histologic alterations after small-bowel transplantation: a comparison of different perfusion solutions. Transplant Proc 24: 1087

    PubMed  CAS  Google Scholar 

  • Thaler W, Oberhuber G, Klima G, Margreiter R, Schmid T (1993) Preservation of small bowel grafts: a comparison with two standard solutions. Eur Surg Res 25: 181

    PubMed  CAS  Google Scholar 

  • Kumar N, Grant D (1999) Small bowel transplantation. Graft 2: 149

    Google Scholar 

  • Sakawaki T, Sasaki K, Hirata K (1998) Saline with glutamine improves cold preserved small bowel graft mortality. Transplantation Proceedings 30: 3471

    Article  PubMed  CAS  Google Scholar 

  • Kaihara S, Egawa H, Inomata Y, Uemoto S, Asonuma K, Tanaka K (1997) Serotonin as a useful parameter for cold and warm ischemic injury in small bowel transplantation. Transplantation 64: 405

    Article  PubMed  CAS  Google Scholar 

  • Hashimoto N, Ohyanagi H (2002) Effect of transplantation on mucosal disaccharidase activity and tissue peptide in a canine small intestine model. Transplant Proc 34: 1013

    Article  PubMed  CAS  Google Scholar 

  • Galmarini D, Ghidoni P, Rossi G, Gatti S, Reggiani P, Bernardi P, Doglia M, Fassati LR (1997) Serotonin (5-HT): an enteroendocrine marker of reperfusion injury after liver-small bowel allotransplantation in the pig. Transplant Proc 3: 1833

    Article  Google Scholar 

  • Larosa CA, Blank MA, Kimura K, Jaffe BM (1990) Effect of transplantation on tissue levels of substance P, vasoactive intestinal polypeptide, and serotonin in rat small intestine. Ann N Y Acad Sci 594: 336

    PubMed  CAS  Google Scholar 

  • Mueler AR, Platz KP, Nusser NC, Heckret C, Schirmeier A, Rayes N, Lang M, Radke P, Neuhaus P (1999) Characterization and Modulation of Preservation/Reperfusion Injury After Small Bowel Transplantation. Transplantation Proceedings 31: 579

    Article  Google Scholar 

  • Deltz E, Thiede A (1985) Microsurgical technique for small bowel transplantation. In: Thiede A et al. (eds) Microsurgical models in rats for transplantation research. Springer, Berlin Heidelberg NewYork: 51

    Google Scholar 

  • Babbs CF (1988) Reperfusion injury of postischemic tissue. Ann Emerg Med 17: 1148

    Article  PubMed  CAS  Google Scholar 

  • Beuk RJ, Heineman E, Tangelder GJ, Josine SLT, Quaedackers W, Marks H, Joshua M, Lieberman M, Egbrink MGA (2000) Total warm ischemia and reperfusion impairs flow in all rat gut layers but increases leukocyte-vessel wall interactions in the submucosa only. Annals of surgery 231: 96

    Article  PubMed  CAS  Google Scholar 

  • Cicalese L, Caraceni P, Nalesnik MA, Borle AB, Schraut WH (1993) Oxygen free radical content and neutrophil infiltration are importatnt determinants in mucosal injury after rat small bowel transplantation. Transplantation 55: 1225

    Article  Google Scholar 

  • Emparan C (1999) Harvest and preservation injury of small bowel grafts in rats: influence of the surgical technique and preservation solution. Eur surg Res 31: 1

    Google Scholar 

  • Hees ML, Manson NH (1984) Molecular oxygen: Friend and foe. The role of the oxygen free radical system in the calcim paradox, the oxygen paradox and ischemia, reperfusion injury. J Mol Cell Cardiol 16: 964

    Google Scholar 

  • Massberg S, Gonzalez AP, Leiderer R, Menger MD, Messmer K (1998) In vivo assessment of the influence of cold preservation time on microvascular reperfusion injury after experimental small bowel transplantation. British Journal of Surgery 85: 127

    Article  PubMed  CAS  Google Scholar 

  • Mueller A, Platz KP, Langrehr JM, Hoffman RA, Nussler AK, Nalesnik M, Billiar TR, Schraut WH (1994) The effect of administration of nitric oxide inhibitors during small bowel preservation and reperfusion. Transplantation 58: 1309

    PubMed  CAS  Google Scholar 

  • Mueller R, Nalesnik M, Platz KP, Langrehr JM, Hoffman RA, Schraut WA (1994) Evaluation of Preservation Conditions and Various Solutions for Small Bowel Preservation. Transplantation 57: 649

    Article  CAS  Google Scholar 

  • Pockley AG, Nakao M, Fairburn B, Wood RFM (2000) Effect of Ischemia-Reperfusion of the Rat Small Bowel on Peripheral Blood Neutrophil CD11b Expression. Tranplantation Proceedings 32: 1304

    Article  CAS  Google Scholar 

  • Taguchi T (1992) Evaluation of UW solution for preservation of small intestine transplants in the rat. Transplantation 53: 1202

    Article  PubMed  CAS  Google Scholar 

  • Baláz P, Matia I, Fronek J, Jackanin S, Rybárová E, Kron I, Pomfy M, Ryska M (2004) Preservation injury of jejunal grafts and its modulation by Custodiol and University of Wisconsin preservation solutions in Wistar rats. Eur Surg Res 802: 1–10

    Google Scholar 

  • Matia I, Baláz P, Jackanin S, Rybárová E, Kron I, Pomfy M, Fronek J, Ryska M (2004) Serotonin levels in the small bowel mucosa as a marker of ischemic injury during small bowel preservation. Ann Transplant 9(3): 48–51

    PubMed  CAS  Google Scholar 

  • Baláz P, Matia I, Jackanin S, Pomfy M, Fronek J, Ryska M (2004) Morphological changes of small bowel graft in Wistar rats after preservation injury. Bratisl Lek Listy 105(2): 62–64

    PubMed  Google Scholar 

  • Park PO, Wallander J, Tufveson G, Haglund U (1991) Cold ischemic and reperfusion injury in a model of small bowel transplantation in the rat. Eur Surg Res 23: 1

    PubMed  CAS  Google Scholar 

  • Chiu CJ, Mcardle AH, Brown R (1970) Intestinal mucosal lesion in low-flow states. Arch Surg 101: 478

    PubMed  CAS  Google Scholar 

  • Sola A, Oca JD, Gonzáles R, Prats N, Roselló-Catafau J, Gelpí E, Jaurrieta E, Hotter G (2000) Protective effect of ischemic preconditioning on cold preservation and reperfusion injury associated with rat intestinal transplantation. Annals of Surgery 234: 98

    Article  Google Scholar 

  • Kaihara S, Hiroto E, Yukihiro I, Shinji U, Katuchiro A, Koicji T (1993) Serotonin as useful parameter for cold and warm ischemic injury in small bowel transplantation. Transplantation 64: 405

    Article  Google Scholar 

  • Balaz P, Kudla, Matia I, Fronek J, Ryska M (2003) Model of small bowel transplantation with systemic venous drainage in rats. Ann Transplant 8(4): 36–38

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Surgery Department, Central Military Hospital, Prague, Czech Republic

    J. Froněk & M. Ryska

  2. Department of Biochemistry and Diagnostics, University Hospital, Hradec Králové, Czech Republic

    P. Živný & J. Vávrová

  3. Currently in Renal Transplant Unit, St George's Hospital, Tooting, UK, London

    J. Froněk

Authors
  1. J. Froněk
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. P. Živný
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. Vávrová
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. Ryska
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to J. Froněk.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Froněk, J., Živný, P., Vávrová, J. et al. Preservation injury of the small bowel graft in rats. Eur Surg 38, 439–444 (2006). https://doi.org/10.1007/s10353-006-0267-3

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10353-006-0267-3

Schlüsselwörter

Keywords

Search

Navigation