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The Effect of Azathioprine on Anastomotic Healing: An Experimental Study in Rats

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  • Published:
Diseases of the Colon & Rectum

Abstract

Purpose

The cytotoxic and immunosuppressive effects of azathioprine, which mitigate the disease activity in inflammatory bowel disease, may compromise the healing of intestinal anastomoses leading to an increased risk of anastomotic leakage. The effect of azathioprine treatment on intestinal healing was tested.

Methods

In an experimental study, rats were randomly given one oral dose of azathioprine (5 mg or 20 mg/kg body weight per day) or placebo. After 28 days of treatment, a left colonic anastomosis was performed. After three days of healing, the breaking strengths of the anastomoses were tested, along with measurements of azathioprine major metabolite concentrations: 6-thioguanine and 6-methyl-mercaptopurine.

Results

There were no significant differences in the anastomotic breaking strength between the three groups.

Conclusions

Daily treatment for four weeks with high or low azathioprine doses has no inhibitory effect on colonic healing in rats.

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Authors and Affiliations

  1. Surgical Department P, Århus Universital Hospital THG, Århus C, Denmark

    Tilo Stolzenburg D.M.Sc., Ken Ljungmann Ph.D & Henrik Christensen D.M.Sc.

  2. Surgical Department P, Århus Sygehus THG, 8000, Århus C, Denmark

    Tilo Stolzenburg D.M.Sc.

Authors
  1. Tilo Stolzenburg D.M.Sc.
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  2. Ken Ljungmann Ph.D
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  3. Henrik Christensen D.M.Sc.
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Additional information

Supported by the Institute for Experimental Clinical Research at Århus University and The Danish Colitis-Crohn Association. The azathioprine compound was provided by GlaxoWellcome Research and Development.

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Stolzenburg, T., Ljungmann, K. & Christensen, H. The Effect of Azathioprine on Anastomotic Healing: An Experimental Study in Rats. Dis Colon Rectum 50, 2203–2208 (2007). https://doi.org/10.1007/s10350-007-9010-9

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  • DOI: https://doi.org/10.1007/s10350-007-9010-9

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