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Diabetes Mellitus and Anal Sphincter Pressures: An Experimental Model in Rats

  • Published:
Diseases of the Colon & Rectum

Purpose

Symptoms of the gastrointestinal tract, frequent in patients with diabetes mellitus, which may be related to an increase in the production of free radicals, include alterations in the function of the sphincter anal musculature. Such alterations are characterized by a decrease of muscular tone associated with different degrees of fecal incontinence. This study was performed to show the alterations in the anal sphincter pressures of diabetic rats and to evaluate the role of nitric oxide and oxidative stress in this situation.

Methods

Male Wistar rats weighing 250 to 400 g were used. The animals were divided in two groups: control and diabetic. Diabetes was induced through intraperitoneal injection of streptozotocin and the anal pressures were gauged by anorectal manometry. Nitric oxide was evaluated through measures of nitrites and nitrates, and oxidative stress through the technique of chemoluminescence.

Results

There was a significant decrease in the sphincter anal pressure of diabetic animals 60 days after induction (P < 0.05). This pressure returned to basal values after administration of a nitric oxide synthase antagonist. The levels of nitrites and nitrates as well as of lipoperoxidation were significantly increased in the diabetic compared with the control group (P < 0.05).

Conclusions

In this study, hyperglycemia of diabetes mellitus caused an increase in the oxidative stress. Apparently the elevation of nitric oxide levels was one of the responsible factors for the decrease of anal sphincter pressures.

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Acknowledgments

The authors thank Dr. Indru Kubchandani and Dr. José Alfredo dos Reis Netto for assistance.

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Authors and Affiliations

  1. Laboratory of Digestive Physiology, Institute of Basic Health Sciences, Federal University of Rio Grande Do Sul, Lutheran University of Brazil RS, Porto Alegre–RS, Brazil

    Henrique Sarubbi Fillmann M.D., Suzana Llessuy Ph.D., Cláudio A. Marroni M.D., Ph.D., Lúcio S. Fillmann M.D., Ph.D. & Norma Possa Marroni Ph.D.

  2. Av. Ipiranga 6690/307, Cep 90610-000, Porto Alegre–RS, Brazil

    Henrique Sarubbi Fillmann M.D.

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  1. Henrique Sarubbi Fillmann M.D.
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  2. Suzana Llessuy Ph.D.
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Correspondence to Henrique Sarubbi Fillmann M.D..

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Fillmann, H.S., Llessuy, S., Marroni, C.A. et al. Diabetes Mellitus and Anal Sphincter Pressures: An Experimental Model in Rats. Dis Colon Rectum 50, 517–522 (2007). https://doi.org/10.1007/s10350-006-0838-1

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  • DOI: https://doi.org/10.1007/s10350-006-0838-1

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