Abstract
PURPOSE: This study was designed to determine the clinical impact of carcinoembryonic antigen messenger ribonucleic acid expression in tumor-draining vein blood on postoperative liver metastasis in patients with colorectal carcinoma. METHODS: The study comprised 37 patients with colorectal carcinoma who underwent laparotomy (Dukes A, 3; Dukes B, 16; Dukes C, 15; and Dukes D, 3). Blood samples were drawn from the tumor-draining vein and the tumor-feeding artery. Total ribonucleic acid was extracted from the blood and subjected to reverse transcriptase-polymerase chain reaction to detect carcinoembryonic antigen messenger ribonucleic acid. Tissue sections of tumor were stained with hematoxylin and eosin and were examined immunohistochemically. The duration of follow-up ranged from two years and three months to three years and six months. RESULTS: Expression of amplified carcinoembryonic antigen messenger ribonucleic acid in tumor-draining vein blood was positive in 16 (43 percent) of the 37 patients, while that in the feeding artery blood was positive in 3 patients (8 percent). There was no relation between the protein level of carcinoembryonic antigen and positive expression of carcinoembryonic antigen messenger ribonucleic acid. Positive expression of carcinoembryonic antigen messenger ribonucleic acid was observed in two patients with histologic evidence of massive venous invasion and in all three patients with synchronous liver metastasis. Immunohistochemical studies revealed that all tumor specimens with stromal type (5/5) of carcinoembryonic antigen staining had positive expression of carcinoembryonic antigen messenger ribonucleic acid. Postoperative liver metastasis was detected in four patients, three of whom (75 percent) had positive expression of carcinoembryonic antigen messenger ribonucleic acid in tumor-draining vein blood. CONCLUSIONS: Positive expression of carcinoembryonic antigen messenger ribonucleic acid in tumor-draining vein blood differed by the type of immunohistochemical staining with carcinoembryonic antigen. These data suggest that patients with positive expression of carcinoembryonic antigen messenger ribonucleic acid in tumor-draining vein blood may be at a high risk for postoperative liver metastasis.
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References
CC Boring TS Squires T Tong (1993) ArticleTitleCancer Statistics 1993 CA Cancer J Clin 43 7–26
IJ Fidler (1990) ArticleTitleCritical factors in the biology of human cancer metastasis Cancer Res 50 6130–6138
BI Eisenstein (1990) ArticleTitleThe polymerase chain reaction. A new method of using molecular genetics for medical diagnosis N Engl J Med 322 178–182
TJ Pelkey HF Frierson SuffixJr DE Bruns (1996) ArticleTitleMolecular and immunological detection of circulating tumor cells and micrometastases from solid tumors Clin Chem 42 1369–1381
P Brossart JW Schmier S Kruger et al. (1995) ArticleTitleA polymerase chain reaction-based semiquantitative assessment of malignant melanoma cells in peripheral blood Cancer Res 55 4065–4068
M Mori K Mimori H Inoue et al. (1995) ArticleTitleDetection of cancer micrometastases in lymph nodes by reverse transcriptase polymerase chain reaction Cancer Res 55 3417–3420
M Gerhard H Juhl H Halthoff et al. (1994) ArticleTitleSpecific detection of carcinoembryonic antigen-expressing tumor cells in bone marrow aspirates by polymerase chain reaction J Clin Oncol 12 725–729
P Gold SO Freedman (1965) ArticleTitleDemonstration of tumor-specific antigen in human colonic carcinoma by immunological tolerance and absorption techniques J Exp Med 121 439–462
G Steele SuffixJr N Zamcheck (1985) ArticleTitleThe use of carcinoembryonic antigen in the clinical management of patients with colorectal cancer Cancer Detect Prev 8 421–427
RB Turnbull SuffixJr K Kyle FR Watson J Spratt (1966) ArticleTitleCancer of the colon Ann Surg 4 99–111
K Sunouchi TA Hamilton CS Tannenbaum RR Tubbs R Bukowski JH Finke (1994) ArticleTitleChemokine gene expression in the murine renal cell carcinoma, RENCA, following treatment in vivo with interferon-α and interleukin-2 Am J Pathol 144 747–755
Y Hamada M Yamamura K Hioki M Yamamoto H Nagura K Watanabe (1985) ArticleTitleCEA distribution pattern and the CEA levels in the peripheral blood in patients with colorectal carcinoma Cancer 55 136–141
K Yamaguchi Y Takagi S Aoki M Futamura S Saji (2000) ArticleTitleSignificant detection of circulating cancer cells in the blood by reverse transcriptase-polymerase chain reaction during colorectal cancer resection Ann Surg 232 58–65
Y Tabuchi T Nakamura T Kuniyasu (1998) ArticleTitleA predictive value of carcinoembryonic antigen levels in draining venous blood for colorectal cancer patients with postoperative hematogenous metastases Cancer Detect Prev 22 57–61
S Fujita K Sugano N Fukayama Y Moriya K Sugihara T Akasu (1996) ArticleTitleDetection of K-ras point mutations in mesenteric venous blood from colorectal cancer patients by enriched polymerase chain reaction and single-strand conformation polymorphism analysis Jpn J Clin Oncol 26 417–421
JC Kim MS Han HK Lee et al. (1999) ArticleTitleDistribution of carcinoembryonic antigen and biologic behavior in colorectal carcinoma Dis Colon Rectum 42 640–648
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Sunouchi, K., Machinami, R., Mori, M. et al. Clinical Impact of Carcinoembryonic Antigen Messenger Ribonucleic Acid Expression in Tumor-Draining Vein Blood on Postoperative Liver Metastasis in Patients With Colorectal Carcinoma. Dis Colon Rectum 46, 467–473 (2003). https://doi.org/10.1007/s10350-004-6584-3
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DOI: https://doi.org/10.1007/s10350-004-6584-3