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European Journal of Wildlife Research

, Volume 62, Issue 6, pp 681–685 | Cite as

Molecular characterisation and serotyping of Pasteurella multocida isolates from Asiatic elephants (Elephas maximus)

  • Basavegowdanadoddi Marinaik ChandranaikEmail author
  • Beechagondahalli Papanna Shivashankar
  • Papanna Giridhar
  • Doddegowdanakoppalu Nanjegowda Nagaraju
Original Article

Abstract

We report the cultural and molecular characterisation of Pasteurella multocida isolates recovered from Asiatic elephants at Bandipur tiger reserve forest in Karnataka state, India. The forest had witnessed consecutive droughts from 2010 to 2012 and sudden heavy monsoon rains in 2013. Twenty-three elephants died during this period of heavy rains between May and July 2013. Postmortem conducted on a fresh elephant carcass revealed hemorrhagic tracheitis, haemorrhages on heart and lesions of acute septicaemia in all other vital organs. In seven elephant carcasses where fresh samples were not available, bone marrow samples of femur bones were aseptically collected. Heart blood and spleen samples collected at postmortem and two out of seven bone marrow samples yielded the growth of P. multocida. The isolates were typed as P. multocida type B. The phylogenetic analysis of the isolates was carried out by 16S ribosomal RNA (rRNA) gene-based PCR and sequencing of 1.3 Kbp nucleotides on the 16S rRNA gene of the isolates. The isolates showed highest sequence identity with P. multocida isolates of bovine and caprine origin. This is the first report of molecular study on P. multocida from Asiatic elephants.

Keywords

Asiatic elephants Serotyping Pasteurella multocida 

References

  1. Anina BJS, Richard KH, Peter K, Bożena MK (2009) Phenotypic and genetic characterization of Pasteurella Multocida and related isolates from rabbits in Switzerland. J Vet Diag Invest 21:793–802CrossRefGoogle Scholar
  2. Baskaran N, Arivazhagan C, Varma S, Sukuma R (2008) Management plan for the elephants in Bandipur tiger reserve. Dissertation, Indian Institute of Science. IndiaGoogle Scholar
  3. Chandranaik BM, Raveendra H, Shivashankar BP, Giridhar P, Muniyellappa HK, Kalge RS, Sumathi BR, Nithinprabhu K, Chandrashekara N, Manjunatha V, Nirupama J, Mayanna A, Chandrakala GK, Kanaka S, Venkatesha MD (2015) Serotyping of Foot and Mouth Disease virus and Pasteurella multocida isolates from Indian Gaurs (Bos gaurus) concurrently infected with Foot and Mouth Disease and Haemorrhagic Septicaemia. Trop Anim Health Prod 47:933–937CrossRefPubMedGoogle Scholar
  4. Chandranaik BM, Giridhar P, Venkatesha MD, Rathnamma D (2016) Monitoring of quality of water in major waterholes in Bandipur and Nagarahole forests. Dissertation, Karnataka Veterinary Animal and Fisheries Sciences University. IndiaGoogle Scholar
  5. Harish BR, Shivaraj CBM, Venkatesha MD, Renukaprasad C (2009) Hemorrhagic septicemia in asian elephants (Elephas maximus) in Karnataka state India. J Threatened Taxa 1:194–195CrossRefGoogle Scholar
  6. OIE (2008) Haemorrhagic septicaemia. In: Manual for diagnostic procedures for terrestrial animal disease. Chapter 2.4.12., pp 739–750Google Scholar
  7. Quinn PJ, Markey BK, Leonard FC, Fitzpatrick ES, Fanning S, Hartigan PJ (2011) Veterinary microbiology and microbial diseases, 2nd edn. Wiley-Blackwell publications, United KingdomeGoogle Scholar
  8. Robert DL (2004) Genetic diversity of Pasteurella multocida strains of avian, bovine, ovine and porcine origin from England and Wales by comparative sequence analysis of 16S rRNA gene. Microbiol 150:4194–4210Google Scholar
  9. Srivatsava AB, Chaturvedi VK (2000) A case of pasteurellosis in elephant. Ind J Comp Microbiol Infect Dis 21:159–160Google Scholar
  10. Sumathi, Vasanthaiah MS, Raju H (2013) Annual seasonal rainfall and area overage during 2012. In Karnataka. Dissertation, Directorate of Economics and Statistics, Government of Karnataka. IndiaGoogle Scholar
  11. Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596–1599CrossRefPubMedGoogle Scholar
  12. Thomson GR, Vosloo W, Bastos ADS (2003) Foot and mouth disease in wildlife. Virus Res 91:145–161CrossRefPubMedGoogle Scholar
  13. Townsend MT, Frost AJ, Lee CW, Papadimitriou JM, Dawkins HJS (1998) Development of PCR Assays for Species- and Type-Specific Identification of Pasteurella multocida Isolates. J Clin Microbiol 36:1096–1100PubMedPubMedCentralGoogle Scholar
  14. Varma S, Sukumar R (2012) Synchronized population estimation of the Asian elephants in forest divisions of Karnataka. Dissertation, Indian Institute of ScienceGoogle Scholar
  15. Venkataramanan R, Bandyopadhyay SK, Oberoi MS (2005) Present status and strategies for the control of transboundary and other economically important animal diseases in India: a review. Ind J Anim Sci 75:456–464Google Scholar
  16. Wickremasuriya UGJS, Kendaragama KMT (1982) A case report of haemorrhagic septicaemia in a wild elephant. Sri Lanka Vet J 30:34Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Basavegowdanadoddi Marinaik Chandranaik
    • 1
    • 3
    Email author
  • Beechagondahalli Papanna Shivashankar
    • 1
  • Papanna Giridhar
    • 1
  • Doddegowdanakoppalu Nanjegowda Nagaraju
    • 2
  1. 1.Institute of Animal Health and Veterinary BiologicalsKarnataka Veterinary and Animal and Fisheries Sciences UniversityBangaloreIndia
  2. 2.Bandipur Tiger ReserveBandipur, Chamarajanagar DistrictIndia
  3. 3.Institute of Animal Health and Veterinary BiologicalsBangaloreIndia

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