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Development of twelve novel polymorphic microsatellite DNA markers for the Boeseman’s rainbowfish (Melanotaenia boesemani) and tests for their cross-utility in 21 rainbowfish species from West Papua (Indonesia)

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Abstract

We developed 12 microsatellite markers for the endangered Boeseman’s rainbowfish (Melanotaenia boesemani). Twenty-eight individuals from the type locality at Ayamaru Lake were examined, and all loci were polymorphic with a number of alleles per locus varying from 3 to 18. Average observed and expected heterozygosities were 0.681 and 0.678, respectively. Cross-species amplification was successfully obtained for 21 Melanotaenia species, with a number of alleles per locus ranging between 1 and 20. Average observed and expected heterozygosities varied between 0.105 and 0.708 and 0.118–0.755, respectively. Only 21 inbreeding coefficient (Fis) values presented a significant homozygote excess among the 264 locus-by-locus calculated values. Tests for genotyping errors revealed that four of these 21 significant Fis values could be explained by the presence of null alleles. These new microsatellite markers appear highly reliable for further conservation purposes or population genetic studies of the many rainbowfish endangered species.

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Acknowledgments

The PhD thesis of Mrs Nugraha is jointly support by the French Ministry of Foreign Affairs and IRD. Data presented in this work were partly produced through the technical facilities of the labex “Centre Méditerranéen de l’Environnement et de la Biodiversité.” This is publication IRD-DIVA-ISEM-2014-160.

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Authors and Affiliations

  1. Institut des Sciences de l’Evolution de Montpellier, UMR 226 IRD-CNRS-UM2, Place Eugène Bataillon, CC065, 34095, Montpellier Cedex 5, France

    Media Fitri Isma Nugraha, Laurent Pouyaud & Jean-Christophe Avarre

  2. Research Centre and Development for Ornamental Fish, Indonesian Agency for Marine and Fisheries Research and Development, Depok, Indonesia

    Media Fitri Isma Nugraha

  3. Department of Aquaculture, Faculty of Fisheries and Marine Science, Bogor Agricultural University (IPB), Bogor, Indonesia

    Odang Carman

  4. Akademi Perikanan Sorong (APSOR), KKD-BP Sumberdaya Genetik dan Konservasi, Sorong, Papua Barat, Indonesia

    Kadarusman

  5. Department of Biology, Faculty of Mathematics and Science, Bogor Agricultural University (IPB), Bogor, Indonesia

    Utut Widyastuti

Authors
  1. Media Fitri Isma Nugraha
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  2. Laurent Pouyaud
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  3. Odang Carman
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  4. Kadarusman
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  5. Utut Widyastuti
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  6. Jean-Christophe Avarre
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Corresponding author

Correspondence to Jean-Christophe Avarre.

Additional information

Communicated by C. Gortázar

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Nugraha, M.F.I., Pouyaud, L., Carman, O. et al. Development of twelve novel polymorphic microsatellite DNA markers for the Boeseman’s rainbowfish (Melanotaenia boesemani) and tests for their cross-utility in 21 rainbowfish species from West Papua (Indonesia). Eur J Wildl Res 60, 941–946 (2014). https://doi.org/10.1007/s10344-014-0868-2

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  • DOI: https://doi.org/10.1007/s10344-014-0868-2

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